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Literature DB >> 31611353

Pyomelanin Secretion in Madurella mycetomatis Interferes with Spectrophotometric Endpoint Reading Using the Sensititre YeastOne alamarBlue Assay but Not with Visual Endpoint Reading.

B Nyuykonge¹, P D Croughs¹, A H Fahal², A Verbon¹, W W J van de Sande³.

Abstract

The use of the Sensititre YeastOne YO10 alamarBlue assay for the in vitro susceptibility testing of Madurella mycetomatis was evaluated in M. mycetomatis isolates with and without pyomelanin secretion. Pyomelanin secretion did not influence visual endpoint reading; however, it caused a shift in peak absorbance from 570 nm to 620 nm when read spectrophotometrically. Therefore, when choosing the method for endpoint reading, the presence of pyomelanin should be considered.

Entities: Chemical Species

Keywords: Madurella mycetomatiszzm321990; azole; melanin; mycetoma; pigment; pyomelanin; susceptibility testing

Year: 2019 PMID： 31611353 PMCID： PMC7187617 DOI： 10.1128/AAC.01532-19

Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN： 0066-4804 Impact factor: 5.191

25 in total

Review 1. Current concepts in laboratory testing to guide antimicrobial therapy.

Authors: Stephen G Jenkins; Audrey N Schuetz
Journal: Mayo Clin Proc Date: 2012-03 Impact factor: 7.616

2. Mycetoma: an old and still neglected tropical disease.

Authors: Roderick J Hay; Ahmed H Fahal
Journal: Trans R Soc Trop Med Hyg Date: 2015-03 Impact factor: 2.184

3. Testing of the in vitro susceptibilities of Madurella mycetomatis to six antifungal agents by using the Sensititre system in comparison with a viability-based 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5- [(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) assay and a modified NCCLS method.

Authors: Wendy W J van de Sande; Ad Luijendijk; Abdalla O A Ahmed; Irma A J M Bakker-Woudenberg; Alex van Belkum
Journal: Antimicrob Agents Chemother Date: 2005-04 Impact factor: 5.191

4. Comparison of visual 24-hour and spectrophotometric 48-hour MICs to CLSI reference microdilution MICs of fluconazole, itraconazole, posaconazole, and voriconazole for Candida spp.: a collaborative study.

Authors: A Espinel-Ingroff; F Barchiesi; M Cuenca-Estrella; A Fothergill; M A Pfaller; M Rinaldi; J L Rodriguez-Tudela; P E Verweij
Journal: J Clin Microbiol Date: 2005-09 Impact factor: 5.948

5. Spectroelectrochemical Reverse Engineering DemonstratesThat Melanin's Redox and Radical Scavenging Activities Are Linked.

Authors: Eunkyoung Kim; Mijeong Kang; Tanya Tschirhart; Mackenzie Malo; Ekaterina Dadachova; Gaojuan Cao; Jun-Jie Yin; William E Bentley; Zheng Wang; Gregory F Payne
Journal: Biomacromolecules Date: 2017-11-01 Impact factor: 6.988

6. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity.

Authors: J O'Brien; I Wilson; T Orton; F Pognan
Journal: Eur J Biochem Date: 2000-09

7. Microplate Alamar blue assay for susceptibility testing of Candida albicans biofilms.

Authors: Kimberly K Repp; Scott A Menor; Robin K Pettit
Journal: Med Mycol Date: 2007-11 Impact factor: 4.076

Review 8. Closing the mycetoma knowledge gap.

Authors: Wendy van de Sande; Ahmed Fahal; Sarah Abdalla Ahmed; Julian Alberto Serrano; Alexandro Bonifaz; Ed Zijlstra
Journal: Med Mycol Date: 2018-04-01 Impact factor: 4.076

Pyomelanin Secretion in Madurella mycetomatis Interferes with Spectrophotometric Endpoint Reading Using the Sensititre YeastOne alamarBlue Assay but Not with Visual Endpoint Reading.

Review 1. Current concepts in laboratory testing to guide antimicrobial therapy.

2. Mycetoma: an old and still neglected tropical disease.

3. Testing of the in vitro susceptibilities of Madurella mycetomatis to six antifungal agents by using the Sensititre system in comparison with a viability-based 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5- [(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) assay and a modified NCCLS method.

4. Comparison of visual 24-hour and spectrophotometric 48-hour MICs to CLSI reference microdilution MICs of fluconazole, itraconazole, posaconazole, and voriconazole for Candida spp.: a collaborative study.

5. Spectroelectrochemical Reverse Engineering DemonstratesThat Melanin's Redox and Radical Scavenging Activities Are Linked.

6. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity.

7. Microplate Alamar blue assay for susceptibility testing of Candida albicans biofilms.

Review 8. Closing the mycetoma knowledge gap.

9. Aspergillus fumigatus melanins: interference with the host endocytosis pathway and impact on virulence.

Review 10. Global burden of human mycetoma: a systematic review and meta-analysis.

1. In vitro susceptibility testing for black grain eumycetoma causative agents.

2. Development and Validation of an In Vitro Resazurin-Based Susceptibility Assay against Madurella mycetomatis.

3. Diagnostic implications of mycetoma derived from Madurella pseudomycetomatis isolates from Mexico.