Chun Feng1, Junjun She2, Xiaobing Chen3, Qunchao Zhang1, Xu Zhang1, Yongsheng Wang4, Jiahui Ye5, Jiajun Shi5, Jinqiu Tao5, Min Feng5, Wenxian Guan5, Hongping Xia2,6, Weijie Zhang5, Guifang Xu7. 1. Department of Gastroenterology, The Third People's Hospital of Bengbu, Bengbu, 233000, PR China. 2. Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, PR China. 3. Department of Oncology, Henan Cancer Hospital, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, PR China. 4. Department of Respiratory Medicine, Nanjing Drum Tower Hospital affiliated to Medical School of Nanjing University, Nanjing 210008, PR China. 5. Department of General Surgery, Nanjing Drum Tower Hospital affiliated to Medical School of Nanjing University, Nanjing 210008, PR China. 6. Department of Pathology, School of Basic Medical Sciences & The Affiliated Sir Run Run Hospital & State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, PR China. 7. Department of Gastroenterology, Nanjing Drum Tower Hospital affiliated to Medical School of Nanjing University, Nanjing 210008, PR China.
Abstract
Aim: To investigate the role of exosomal miRNAs on gastric cancer (GC) metastasis. Materials & methods: miRNA expression profiles of exosomes with distinct invasion potentials were analyzed using miRNA microarray and validated by quantitative real-time PCR. In vitro and in vivo experiments assessed the role of exosomal miR-196a-1 in GC's metastasis. Results: High expression level of exosomal miR-196a-1 expression was significantly associated with poor survival in GC. Exosomes that contained miR-196a-1 were secreted from high-invasive GC cells. Ectopic miR-196a-1 expression promoted invasion of low-invasive GC cells by targeting SFRP1. Conclusion: miR-196a-1 was delivered from high-invasive GC into low-invasive GC cells via exosomes and promoted metastasis to the liver in vitro and in vivo.
Aim: To investigate the role of exosomal miRNAs on gastric cancer (GC) metastasis. Materials & methods: miRNA expression profiles of exosomes with distinct invasion potentials were analyzed using miRNA microarray and validated by quantitative real-time PCR. In vitro and in vivo experiments assessed the role of exosomal miR-196a-1 in GC's metastasis. Results: High expression level of exosomal miR-196a-1 expression was significantly associated with poor survival in GC. Exosomes that contained miR-196a-1 were secreted from high-invasive GC cells. Ectopic miR-196a-1 expression promoted invasion of low-invasive GC cells by targeting SFRP1. Conclusion:miR-196a-1 was delivered from high-invasive GC into low-invasive GC cells via exosomes and promoted metastasis to the liver in vitro and in vivo.