The responses of human gingival fibroblasts to magnesium-doped titanium.

The titanium (Ti) implant is widely used in implant dentistry; yet peri-implantitis has always been one of the most common and serious complications. Here, we demonstrated that magnesium-doping would be an effective way of enhancing the integration between implant surfaces and gingival tissues, which is critical to peri-implant health. The magnesium (2.76-6.35 at %) was immobilized onto the titanium substrate by a magnesium plasma immersion ion implantation (Mg-PIII) technique. Mg-PIII treatments did not alter surface topographies of the original titanium substrate but improved its hydrophilicity. The in vitro study including cell viability, adhesion, proliferation, migration, and real-time polymerase chain reaction assays disclosed improved adhesion, proliferation, migration, and extracellular matrix remodeling abilities of human gingival fibroblasts (HGFs) on the magnesium-doped titanium. The results of western blot suggested that the Mg-modified titanium induced the phosphorylation of AKT through the activation of PI3K. Our results revealed that magnesium-doping would potentially enhance soft tissue sealings by promoting cellular functions of HGFs in a dose-dependent manner, boding well for its applications on surfaces of implant necks in early peri-implant soft tissue integrations.