Che Ghazali Norul Hajar1, Zefarina Zulkafli2, Nor Suhaila Md Riffin3, Tuan Hulwani Tuan Mohammad4, Sabreena Safuan5, Bryan Raveen Nelson6, Mohd Tajuddin Abdullah6, Geoffrey Keith Chambers7, Hisham Atan Edinur8. 1. School of Health Sciences, Universiti Sains Malaysia, Kelantan, Malaysia; Hospital Universiti Sains Malaysia, Universiti Sains Malaysia, Kelantan, Malaysia. 2. Hospital Universiti Sains Malaysia, Universiti Sains Malaysia, Kelantan, Malaysia; School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia. 3. Hospital Sultan Haji Ahmad Shah, 28000, Temerloh, Pahang Darul Makmur, Malaysia. 4. Hospital Seberang Jaya, Perai, Pulau Pinang, Malaysia. 5. School of Health Sciences, Universiti Sains Malaysia, Kelantan, Malaysia. 6. Institute of Tropical Biodiversity and Sustainable Development, Universiti Malaysia Terengganu, Terengganu, Malaysia. 7. School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand. 8. School of Health Sciences, Universiti Sains Malaysia, Health Campus, Kelantan, Malaysia. Electronic address: edinur@usm.my.
Abstract
BACKGROUND: Human neutrophil antigens (HNAs) are implicated in several clinical disorders and their allelic variations have been reported for many populations. This new study was aimed to report the genotype and alleles frequencies of HNA-1, -3, -4 and -5 loci in Malays, Chinese and Indians in Peninsular Malaysia. METHODS: A total of 222 blood samples were collected from healthy, unrelated Malay, Chinese and Indian individuals. Their HNA-1, -3 and -4 and HNA-5 loci were genotyped using polymerase chain reaction-sequence specific primer (PCR-SSP) or PCR-restriction fragment length polymorphism (RFLP) assays. RESULTS: All HNA loci are polymorphic, except for HNA -4. Geneotypes HNA-1a/1b, -3a/3b and -4a/4a were observed most frequently at these three loci in all three ethnic groups. In contrast, HNA-5a/5b and -5a/5a were observed as the predominant genotypes in Malays vs. Chinese and Indians, respectively. The Malays, Chinese and Indians shared HNA -3a (0.505-0.527), HNA -4a (1.000) and -5a (0.676-0.854) as the most frequent alleles. However, HNA-1a was found to be the most common in Malays (0.506) and Chinese (0.504) and HNA-1b for Indians (0.525). CONCLUSION: Combined with HNA data that have been published for Malay subethnic and Orang Asli groups, this study provides the first fully comprehensive HNA dataset for populations to be found in Peninsular Malaysia. Overall, our findings provide further evidence of genetic complexity in the region. This now publicly available HNA dataset can be used as a reliable reference source for improving medical outcomes.
BACKGROUND:Human neutrophil antigens (HNAs) are implicated in several clinical disorders and their allelic variations have been reported for many populations. This new study was aimed to report the genotype and alleles frequencies of HNA-1, -3, -4 and -5 loci in Malays, Chinese and Indians in Peninsular Malaysia. METHODS: A total of 222 blood samples were collected from healthy, unrelated Malay, Chinese and Indian individuals. Their HNA-1, -3 and -4 and HNA-5 loci were genotyped using polymerase chain reaction-sequence specific primer (PCR-SSP) or PCR-restriction fragment length polymorphism (RFLP) assays. RESULTS: All HNA loci are polymorphic, except for HNA -4. Geneotypes HNA-1a/1b, -3a/3b and -4a/4a were observed most frequently at these three loci in all three ethnic groups. In contrast, HNA-5a/5b and -5a/5a were observed as the predominant genotypes in Malays vs. Chinese and Indians, respectively. The Malays, Chinese and Indians shared HNA -3a (0.505-0.527), HNA -4a (1.000) and -5a (0.676-0.854) as the most frequent alleles. However, HNA-1a was found to be the most common in Malays (0.506) and Chinese (0.504) and HNA-1b for Indians (0.525). CONCLUSION: Combined with HNA data that have been published for Malay subethnic and Orang Asli groups, this study provides the first fully comprehensive HNA dataset for populations to be found in Peninsular Malaysia. Overall, our findings provide further evidence of genetic complexity in the region. This now publicly available HNA dataset can be used as a reliable reference source for improving medical outcomes.
Authors: Che Ghazali Norul Hajar; Zulkafli Zefarina; Nor Suhaila Md Riffin; Tuan Hulwani Tuan Mohammad; Mohd Nazri Hassan; Ameer Mohamed Dafalla; Gehad ElGhazali; Geoffrey Keith Chambers; Hisham Atan Edinur Journal: Ann Lab Med Date: 2020-06-17 Impact factor: 3.464