Fernanda F Juliano1, José Fernando R Alvarenga2, Rosa Maria Lamuela-Raventos3, Adna P Massarioli1, Liziane M Lima4, Roseane C Santos4, Severino M Alencar1. 1. Department of Agri-food Industry, Food and Nutrition, 'Luiz de Queiroz' College of Agriculture, University of São Paulo, Piracicaba, Brazil. 2. Department of Food and Nutrition, School of Pharmaceutical Sciences, São Paulo State University-UNESP, Araraquara, Brazil. 3. Department of Nutrition, Food Sciences and Gastronomy, XARTA, INSA-UB, School of Pharmacy and Food Sciences, University of Barcelona, Carrer de Prat de la Riba, Santa Coloma de Gramenet, Spain. 4. Embrapa Cotton, Brazilian Agricultural Research Corporation, Campina Grande, Brazil.
Abstract
BACKGROUND: Peanut (Arachis hypogaea L.) is an important economic food crop highly appreciated worldwide. Although peanut cultivation has been expanding to dry regions, with water stress during growth stages, current genotypes are not adapted to drought. This study aimed to identify and quantify the full range of polyphenols in five peanut genotypes with different degrees of drought tolerance developed by Embrapa (Brazil) using ultra-high-definition accurate-mass liquid chromatography-electrospray ionization-LTQ-Orbitrap-mass spectrometry. RESULTS: Fifty-eight polyphenols of the classes hydroxybenzoic acids, hydroxycinnamic acids, flavanols, flavonols and flavanones were identified and quantified using high mass accuracy data and confirmed by tandem mass spectrometric experiments. High-definition mass spectrometric analyses revealed nine phenolic compounds that have never been reported in peanuts before. Polyphenol assessment using multivariate analysis allowed identification of the level of similarities among the five peanut genotypes studied. CONCLUSION: Higher drought-tolerant genotypes exhibited higher content of flavonoids, which suggests a relationship between these compounds and drought tolerance traits.
BACKGROUND:Peanut (Arachis hypogaea L.) is an important economic food crop highly appreciated worldwide. Although peanut cultivation has been expanding to dry regions, with water stress during growth stages, current genotypes are not adapted to drought. This study aimed to identify and quantify the full range of polyphenols in five peanut genotypes with different degrees of drought tolerance developed by Embrapa (Brazil) using ultra-high-definition accurate-mass liquid chromatography-electrospray ionization-LTQ-Orbitrap-mass spectrometry. RESULTS: Fifty-eight polyphenols of the classes hydroxybenzoic acids, hydroxycinnamic acids, flavanols, flavonols and flavanones were identified and quantified using high mass accuracy data and confirmed by tandem mass spectrometric experiments. High-definition mass spectrometric analyses revealed nine phenolic compounds that have never been reported in peanuts before. Polyphenol assessment using multivariate analysis allowed identification of the level of similarities among the five peanut genotypes studied. CONCLUSION: Higher drought-tolerant genotypes exhibited higher content of flavonoids, which suggests a relationship between these compounds and drought tolerance traits.
Authors: Adna P Massarioli; Alan G de O Sartori; Fernanda F Juliano; Roseane C Dos Santos; Jean Pierre C Ramos; Liziane Maria de Lima; Severino Matias de Alencar Journal: Foods Date: 2022-02-03