| Literature DB >> 31602615 |
Romanas Chaleckis1,2, Kazuto Ohashi3, Isabel Meister4,5, Shama Naz5, Craig E Wheelock4,5.
Abstract
Liquid chromatography-mass spectrometry (LC-MS) based nontargeted metabolomics has been applied to a wide range of biological samples and can provide information on thousands of compounds. However, reliable identification of the compounds remains a challenge affecting result interpretation. In this protocol, we describe comparable yeast cell and whole blood metabolome sample preparation for extracting similar compound groups, and we present a LC-MS method using the all ion fragmentation (AIF) approach for the purposes of increasing accuracy in metabolite annotation. Our method enables database-dependent targeted as well as nontargeted metabolomics analysis from the same data acquisition, while simultaneously improving the accuracy in metabolite identification to increase the quality of the resulting biological information.Entities:
Keywords: All ion fragmentation (AIF); Liquid chromatography–mass spectrometry (LC-MS); Metabolite annotation; Metabolomics
Mesh：
Year: 2019 PMID： 31602615 DOI： 10.1007/978-1-4939-9736-7_14
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745