Felipe Faria Pierotti1, Carolina Sanchez Aranda2, Renata Rodrigues Cocco1, Emanuel Sarinho3, Flávio Sano4, Arnaldo Porto5, Nelson Rosário6, Herberto José Chong Neto6, Ekaterini Goudouris7, Lilian Sanches Moraes8, Neusa Falbo Wandalsen9, Márcia Carvalho Mallozi9, Antônio Carlos Pastorino10, Jackeline Motta Franco11, Maria Letícia Chavarria12, Magnus P Borres13, Dirceu Solé14. 1. Federal University of São Paulo, São Paulo, SP, Brazil. 2. Federal University of São Paulo, São Paulo, SP, Brazil. Electronic address: carolaaranda@yahoo.com.br. 3. Federal University of Pernambuco, Pernambuco, Brazil. 4. Nipo-Brasileiro Hospital, São Paulo, Brazil. 5. University of Passo Fundo, Rio Grande do Sul, Brazil. 6. Federal University of Paraná, Paraná, Brazil. 7. Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. 8. Federal University of Mato Grosso, Mato Grosso, Brazil. 9. Medical University of Santo André, São Paulo, Brazil. 10. University of São Paulo, São Paulo, Brazil. 11. Federal University of Sergipe, Sergipe, Brazil. 12. Federal University of Goias, Goiás, Brazil. 13. Uppsala University, Women's and Children's Health, Uppsala, Sweden and Thermo Fisher Scientific, Uppsala, Sweden. 14. Federal University of São Paulo, São Paulo, SP, Brazil. Electronic address: sole.dirceu@gmail.com.
Abstract
The clinical history is of importance in the investigation of allergic diseases but does have limitations. Many allergic conditions will be over-diagnosed if anamnesis alone is used for diagnostic criteria. Serum total immunoglobulin E (TIgE) quantification, as well as panels containing allergens prevalent in the studied population, may serve as screening tests and facilitate the diagnosis of allergic disease or its exclusion. We assessed the positivity of two versions of these tests, Phadiatop Europe® (PhEU) and Phadiatop Infant® (PhInf), as well as total IgE (TigE) values in patients with a medical diagnosis of allergic disease and non-allergic individuals. METHODS: A cross-sectional study performed in eleven Brazilian pediatric allergy centers with patients divided into groups according to the primary condition and a group of assessed control subjects. They were submitted to TIgE measurement and screening tests (PhEu and PhInf). RESULTS: TIgE mean serum levels were significantly higher among allergic patients, especially those with asthma/rhinitis or atopic dermatitis. The positivity of the screening tests, considering the total population, was 63.8% for PhEU and 72.6% for PhInf. These increased when we evaluated only the allergic subjects. The concordance index of the two tests was Kappa=0.7 and higher among those of greater age. CONCLUSIONS: In the assessed population, there were significantly higher levels among those with positive screening tests and PhInf showed better performance in the identification of sensitized individuals, regardless of age. This is the first study to evaluate Phadiatop and Phadiatop Infant in the same population.
The clinical history is of importance in the investigation of allergic diseases but does have limitations. Many allergic conditions will be over-diagnosed if anamnesis alone is used for diagnostic criteria. Serum total immunoglobulin E (TIgE) quantification, as well as panels containing allergens prevalent in the studied population, may serve as screening tests and facilitate the diagnosis of allergic disease or its exclusion. We assessed the positivity of two versions of these tests, Phadiatop Europe® (PhEU) and Phadiatop Infant® (PhInf), as well as total IgE (TigE) values in patients with a medical diagnosis of allergic disease and non-allergic individuals. METHODS: A cross-sectional study performed in eleven Brazilian pediatric allergy centers with patients divided into groups according to the primary condition and a group of assessed control subjects. They were submitted to TIgE measurement and screening tests (PhEu and PhInf). RESULTS: TIgE mean serum levels were significantly higher among allergicpatients, especially those with asthma/rhinitis or atopic dermatitis. The positivity of the screening tests, considering the total population, was 63.8% for PhEU and 72.6% for PhInf. These increased when we evaluated only the allergic subjects. The concordance index of the two tests was Kappa=0.7 and higher among those of greater age. CONCLUSIONS: In the assessed population, there were significantly higher levels among those with positive screening tests and PhInf showed better performance in the identification of sensitized individuals, regardless of age. This is the first study to evaluate Phadiatop and Phadiatop Infant in the same population.