OBJECTIVE: The important role of microRNA-1271 (miR-1271) has been identified in human diseases and cancers. However, the biological function of miR-1271 remains ambiguous in papillary thyroid carcinoma (PTC). Therefore, the specific role of miR-1271 was investigated in PTC. PATIENTS AND METHODS: The expressions of miR-1271 and insulin receptor substrate 1 (IRS1) were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) assay. The protein expression of the genes was measured by Western blot analysis. The function of miR-1271 was investigated using methyl thiazolyl tetrazolium (MTT) and transwell assays. The Dual-Luciferase assay was used to observe the relationship between miR-1271 and IRS1. RESULTS: MiR-1271 was downregulated in PTC tissues. Moreover, overexpression of miR-1271 suppressed migration, invasion and proliferation of PTC cells. Furthermore, IRS1 was indicated as a direct target gene of miR-1271 and knockdown of IRS1 inhibited cell migration, invasion and proliferation in PTC. In addition, miR-1271 inhibited the progression of PTC by targeting IRS1. Besides that, miR-1271 blocked the epithelial-mesenchymal transition (EMT) and protein kinase B (AKT) pathway in PTC. CONCLUSIONS: MiR-1271 inhibited the progression of PTC by targeting IRS1 and blocking EMT and AKT pathway.
OBJECTIVE: The important role of microRNA-1271 (miR-1271) has been identified in humandiseases and cancers. However, the biological function of miR-1271 remains ambiguous in papillary thyroid carcinoma (PTC). Therefore, the specific role of miR-1271 was investigated in PTC. PATIENTS AND METHODS: The expressions of miR-1271 and insulin receptor substrate 1 (IRS1) were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) assay. The protein expression of the genes was measured by Western blot analysis. The function of miR-1271 was investigated using methyl thiazolyl tetrazolium (MTT) and transwell assays. The Dual-Luciferase assay was used to observe the relationship between miR-1271 and IRS1. RESULTS:MiR-1271 was downregulated in PTC tissues. Moreover, overexpression of miR-1271 suppressed migration, invasion and proliferation of PTC cells. Furthermore, IRS1 was indicated as a direct target gene of miR-1271 and knockdown of IRS1 inhibited cell migration, invasion and proliferation in PTC. In addition, miR-1271 inhibited the progression of PTC by targeting IRS1. Besides that, miR-1271 blocked the epithelial-mesenchymal transition (EMT) and protein kinase B (AKT) pathway in PTC. CONCLUSIONS:MiR-1271 inhibited the progression of PTC by targeting IRS1 and blocking EMT and AKT pathway.
Authors: Mariusz Rogucki; Iwona Sidorkiewicz; Magdalena Niemira; Janusz Bogdan Dzięcioł; Angelika Buczyńska; Agnieszka Adamska; Katarzyna Siewko; Maria Kościuszko; Katarzyna Maliszewska; Anna Wójcicka; Jakub Supronik; Małgorzata Szelachowska; Joanna Reszeć; Adam Jacek Krętowski; Anna Popławska-Kita Journal: Cancers (Basel) Date: 2022-05-28 Impact factor: 6.575