Literature DB >> 3159799

Lymphocyte subpopulations do not alter during blood storage at 4 degrees C.

R Van Lambalgen, G J Van Meurs.   

Abstract

Recent articles have suggested that lymphocyte subpopulations, as determined by monoclonal antibodies, alter during overnight storage of whole blood at 4 degrees C. In contrast, we report here that the expression of lymphocyte subpopulation markers does not alter, but that aberrant results may be obtained due to a large amount of contaminating granulocytes in the lymphocyte preparation, thus indicating that the 'apparent loss' of lymphocyte markers is a technical rather than a biological problem.

Mesh:

Substances:

Year:  1985        PMID: 3159799     DOI: 10.1016/0022-1759(85)90162-0

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  3 in total

1.  Pediatric solid-organ transplant recipients carry chronic loads of Epstein-Barr virus exclusively in the immunoglobulin D-negative B-cell compartment.

Authors:  C Rose; M Green; S Webber; D Ellis; J Reyes; D Rowe
Journal:  J Clin Microbiol       Date:  2001-04       Impact factor: 5.948

2.  Shipping blood to a central laboratory in multicenter clinical trials: effect of ambient temperature on specimen temperature, and effects of temperature on mononuclear cell yield, viability and immunologic function.

Authors:  Walter C Olson; Mark E Smolkin; Erin M Farris; Robyn J Fink; Andrea R Czarkowski; Jonathan H Fink; Kimberly A Chianese-Bullock; Craig L Slingluff
Journal:  J Transl Med       Date:  2011-03-08       Impact factor: 5.531

3.  Adjustment of Cell-Type Composition Minimizes Systematic Bias in Blood DNA Methylation Profiles Derived by DNA Collection Protocols.

Authors:  Yuh Shiwa; Tsuyoshi Hachiya; Ryohei Furukawa; Hideki Ohmomo; Kanako Ono; Hisaaki Kudo; Jun Hata; Atsushi Hozawa; Motoki Iwasaki; Koichi Matsuda; Naoko Minegishi; Mamoru Satoh; Kozo Tanno; Taiki Yamaji; Kenji Wakai; Jiro Hitomi; Yutaka Kiyohara; Michiaki Kubo; Hideo Tanaka; Shoichiro Tsugane; Masayuki Yamamoto; Kenji Sobue; Atsushi Shimizu
Journal:  PLoS One       Date:  2016-01-22       Impact factor: 3.240

  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.