Öcal Berkan1, Serdal Arslan2, Torkia Lalem3, Lu Zhang3, Nil Özbilum Şahin4, Eylem Itır Aydemir5, Özge Korkmaz6, Hatice Reyhan Eğilmez7, Nilgün Çekin2, Yvan Devaux3. 1. Department of Cardiovascular Surgery, Çiğli Training & Research Hospital, İzmir 35620, Turkey. 2. Department of Medical Biology, Faculty of Medicine, Sivas Cumhuriyet University, Sivas 58140, Turkey. 3. Cardiovascular Research Unit, Luxembourg Institute of Health, Strassen L-1445, Luxembourg. 4. Department of Molecular Biology & Genetics, Faculty of Science, Sivas Cumhuriyet University, Sivas 58140, Turkey. 5. Department of Statistics, Faculty of Science, Sivas Cumhuriyet University, Sivas 58140, Turkey. 6. Department of Cardiovascular Surgery, Koru Hospital, Yüksek İhtisas University, Ankara 06510, Turkey. 7. Department of Pathology Faculty of Medicine, Sivas Cumhuriyet University, Sivas 58140, Turkey.
Abstract
Aim: Identification of microRNAs (miRNAs) associated with atherosclerosis may unravel novel therapeutic targets and biomarkers. We studied miRNAs differentially expressed between coronary atherosclerotic plaques (CAP) and healthy arteries. Materials & methods: Paired CAP and internal mammary arteries (IMA) were collected from 14 coronary artery disease patients. The miRNA profiles between diseased (CAP) and healthy (IMA) tissues were compared using microarrays and quantitative PCR. Results: Thirty-one miRNAs were differentially expressed between CAP and IMA. Among these, miR-486-5p showed a high level of regulation (12-fold), had predicted interactions with atherosclerosis-associated genes and correlated with triglyceride levels and arterial stenosis. Regulation of miR-486-5p was validated by PCR (p = 0.004). Conclusion: The miRNAs are regulated in the atherosclerotic plaque. We highlight miR-486-5p whose role in atherosclerosis requires further investigation.
Aim: Identification of microRNAs (miRNAs) associated with atherosclerosis may unravel novel therapeutic targets and biomarkers. We studied miRNAs differentially expressed between coronary atherosclerotic plaques (CAP) and healthy arteries. Materials & methods: Paired CAP and internal mammary arteries (IMA) were collected from 14 coronary artery diseasepatients. The miRNA profiles between diseased (CAP) and healthy (IMA) tissues were compared using microarrays and quantitative PCR. Results: Thirty-one miRNAs were differentially expressed between CAP and IMA. Among these, miR-486-5p showed a high level of regulation (12-fold), had predicted interactions with atherosclerosis-associated genes and correlated with triglyceride levels and arterial stenosis. Regulation of miR-486-5p was validated by PCR (p = 0.004). Conclusion: The miRNAs are regulated in the atherosclerotic plaque. We highlight miR-486-5p whose role in atherosclerosis requires further investigation.
Authors: Emma Louise Robinson; Clarissa Pedrosa da Costa Gomes; Ines Potočnjak; Jan Hellemans; Fay Betsou; David de Gonzalo-Calvo; Monika Stoll; Mehmet Birhan Yilmaz; Bence Ágg; Dimitris Beis; Maria Carmo-Fonseca; Francisco J Enguita; Soner Dogan; Bilge G Tuna; Blanche Schroen; Wim Ammerlaan; Gabriela M Kuster; Irina Carpusca; Thierry Pedrazzini; Costanza Emanueli; Fabio Martelli; Yvan Devaux Journal: Noncoding RNA Date: 2020-05-18
Authors: Yan Wen; Yao Chun; Zhong Qing Lian; Zhang Wei Yong; Yang Mei Lan; Liao Huan; Chen Yi Xi; Li Shu Juan; Zhong Wen Qing; Cheng Jia; Zhang Huan Ji Journal: Mol Med Rep Date: 2021-03-02 Impact factor: 2.952