Logan Meurer1, William Payne1, J Stephen Guffey2. 1. Department of Clinical Lab Science, Arkansas State University, AR 72467, USA. 2. Department of Physical Therapy, P.O. Box 910, Arkansas State University, AR 72467, USA. Electronic address: jguffey@astate.edu.
Abstract
INTRODUCTION: Campylobacter jejuni is a Gram-negative, microaerophilic bacteria that is the leading cause of food-borne illnesses in the United States; it frequently causes severe diarrhoea. Campylobacter jejuni has recently demonstrated antibiotic resistance; therefore, alternative methods for treating these infections must be investigated. Visible light has proven to be an effective inhibitory agent for many medically significant species of bacteria and fungi. METHODS: Visible light in the blue range (405 nm and 464 nm) was administered to a suspension of Campylobacter jejuni. Brain-heart infusion agar was inoculated with the suspension of the organism following exposure to blue light at a dose of 24 Jcm2 for each wavelength. Inoculated plates were placed into a traditional Campy jar in order to mimic a 5% O2 atmosphere and incubated at 37 °C for 48 hours. Following incubation, colony forming units were counted and compared with controls to determine if the light energy had effectively inhibited the growth of the organism. RESULTS: Both 405 nm and 464 nm light at a dose of 24 Jcm2 were shown to be effective inhibitors of Campylobacter jejuni, with kill rates ranging from 82.4-98.9%. CONCLUSION: Blue light effectively inhibits Campylobacter jejuni.
INTRODUCTION:Campylobacter jejuni is a Gram-negative, microaerophilic bacteria that is the leading cause of food-borne illnesses in the United States; it frequently causes severe diarrhoea. Campylobacter jejuni has recently demonstrated antibiotic resistance; therefore, alternative methods for treating these infections must be investigated. Visible light has proven to be an effective inhibitory agent for many medically significant species of bacteria and fungi. METHODS: Visible light in the blue range (405 nm and 464 nm) was administered to a suspension of Campylobacter jejuni. Brain-heart infusion agar was inoculated with the suspension of the organism following exposure to blue light at a dose of 24 Jcm2 for each wavelength. Inoculated plates were placed into a traditional Campy jar in order to mimic a 5% O2 atmosphere and incubated at 37 °C for 48 hours. Following incubation, colony forming units were counted and compared with controls to determine if the light energy had effectively inhibited the growth of the organism. RESULTS: Both 405 nm and 464 nm light at a dose of 24 Jcm2 were shown to be effective inhibitors of Campylobacter jejuni, with kill rates ranging from 82.4-98.9%. CONCLUSION: Blue light effectively inhibits Campylobacter jejuni.
Authors: Peter Walker; Aidan J Taylor; Andrew Hitchcock; Joseph P Webb; Jeffrey Green; Julia Weinstein; David J Kelly Journal: mSystems Date: 2022-08-04 Impact factor: 7.324