Literature DB >> 31589231

Pyrococcus furiosus Argonaute-mediated nucleic acid detection.

Ruyi He1, Longyu Wang1, Fei Wang1, Wenqiang Li1, Yang Liu1, Aitao Li1, Yuan Wang2, Wuxiang Mao1, Chao Zhai1, Lixin Ma1.   

Abstract

We demonstrate that short single-stranded DNA generated by Pyrococcus furiosus Argonaute (PfAgo) can initiate a second round of cleavage. Based on this principle, we established a molecular diagnostic method, termed PfAgo-mediated Nucleic acid Detection (PAND). This method could detect DNA at attomolar sensitivities, distinguish single-nucleotide mutants and accomplish multiplexed detection.

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Year:  2019        PMID： 31589231     DOI： 10.1039/c9cc07339f

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  9 in total

1.  Loosely-packed dynamical structures with partially-melted surface being the key for thermophilic argonaute proteins achieving high DNA-cleavage activity.

Authors:  Lirong Zheng; Hui Lu; Bing Zan; Song Li; Hao Liu; Zhuo Liu; Juan Huang; Yongjia Liu; Fan Jiang; Qian Liu; Yan Feng; Liang Hong
Journal:  Nucleic Acids Res       Date:  2022-07-22       Impact factor: 19.160

2.  Argonaute integrated single-tube PCR system enables supersensitive detection of rare mutations.

Authors:  Qian Liu; Xiang Guo; Guanhua Xun; Zhonglei Li; Yuesheng Chong; Litao Yang; Hongxia Wang; Fengchun Zhang; Shukun Luo; Li Cui; Pengshu Zhao; Xingyu Ye; Heshan Xu; Hui Lu; Xiao Li; Zixin Deng; Kai Li; Yan Feng
Journal:  Nucleic Acids Res       Date:  2021-07-21       Impact factor: 16.971

3.  A programmable omnipotent Argonaute nuclease from mesophilic bacteria Kurthia massiliensis.

Authors:  Yang Liu; Wenqiang Li; Xiaoman Jiang; Yaping Wang; Zhiwei Zhang; Qi Liu; Ruyi He; Quan Chen; Jun Yang; Longyu Wang; Fei Wang; Lixin Ma
Journal:  Nucleic Acids Res       Date:  2021-02-22       Impact factor: 16.971

4.  PfAgo-based detection of SARS-CoV-2.

Authors:  Fei Wang; Jun Yang; Ruyi He; Xiao Yu; Shuliang Chen; Yang Liu; Longyu Wang; Aitao Li; Linlin Liu; Chao Zhai; Lixin Ma
Journal:  Biosens Bioelectron       Date:  2020-12-28       Impact factor: 10.618

5.  Pyrococcus furiosus Argonaute coupled with modified ligase chain reaction for detection of SARS-CoV-2 and HPV.

Authors:  Longyu Wang; Ruyi He; Bin Lv; Xiao Yu; Yang Liu; Jun Yang; Wenqiang Li; Yuan Wang; Hang Zhang; Guangbo Yan; Wuxiang Mao; Linlin Liu; Fei Wang; Lixin Ma
Journal:  Talanta       Date:  2021-02-11       Impact factor: 6.057

6.  A programmable pAgo nuclease with universal guide and target specificity from the mesophilic bacterium Kurthia massiliensis.

Authors:  Ekaterina Kropocheva; Anton Kuzmenko; Alexei A Aravin; Daria Esyunina; Andrey Kulbachinskiy
Journal:  Nucleic Acids Res       Date:  2021-04-19       Impact factor: 16.971

7.  A programmable pAgo nuclease with RNA target preference from the psychrotolerant bacterium Mucilaginibacter paludis.

Authors:  Wenqiang Li; Yang Liu; Ruyi He; Longyu Wang; Yaping Wang; Wanting Zeng; Zhiwei Zhang; Fei Wang; Lixin Ma
Journal:  Nucleic Acids Res       Date:  2022-05-20       Impact factor: 16.971

8.  Prokaryotic Argonaute Proteins as a Tool for Biotechnology.

Authors:  E V Kropocheva; L A Lisitskaya; A A Agapov; A A Musabirov; A V Kulbachinskiy; D M Esyunina
Journal:  Mol Biol       Date:  2022-08-30       Impact factor: 1.540

9.  A Hyperthermophilic Argonaute From Ferroglobus placidus With Specificity on Guide Binding Pattern.

Authors:  Xiang Guo; Yingying Sun; Liuqing Chen; Fei Huang; Qian Liu; Yan Feng
Journal:  Front Microbiol       Date:  2021-06-09       Impact factor: 5.640
  9 in total

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