Rita Bonfiglio1, Filippo Milano2, Ana Cranga1, Maria Teresa De Caro2, Harpreet Kaur Lamsira2, Donata Trivigno1, Stefania Urso2, Manuel Scimeca3, Elena Bonanno4. 1. Department of Experimental Medicine, University of Rome "Tor Vergata", Rome, Italy. 2. Department of Biomedicine and Prevention, University of Rome "Tor Vergata", Italy. 3. Department of Biomedicine and Prevention, University of Rome "Tor Vergata", Italy; Fondazione Umberto Veronesi (FUV), Piazza Velasca 5, 20122, Milano, Mi, Italy; San Raffaele University, Via di Val Cannuta 247, 00166, Rome, Italy; UniCamillus, Saint Camillus International University of Health Sciences, Rome, Italy. Electronic address: manuel.scimeca@uniroma2.it. 4. Department of Experimental Medicine, University of Rome "Tor Vergata", Rome, Italy; "Diagnostica Medica" and "Villa dei Platani", Avellino, Italy (Neuromed group), Italy.
Abstract
BACKGROUND: Recent studies showed a correlation between Body Mass Index and both breast cancer occurrence and progression. Nevertheless, no study reported an accurate evaluation of intra-ductal fat infiltrate. Therefore, the main aim of this study was to evaluate the putative association between intra-ductal fat infiltrate (IDFi) and breast cancer subtypes by using digital pathology. METHODS: We retrospectively collected 220 breast biopsies. Paraffin serial sections were used for haematoxylin and eosin staining and immunohistochemical evaluation of the following markers: estrogen receptor (ER), progesterone receptor (PR), Ki67 and c-erb2. Three haematoxylin and eosin sections for each paraffin block were digitalized. Digital slides were used to evaluate the areas of IDFi. Five randomized areas were evaluated for each slide. By using GraphPad software IDFi areas was correlated with a) breast cancer histotype, b) presence of microcalcifications and c) biomarkers expression. RESULTS: Breast biopsies were classified as follow: 20 normal breast, 50 benign lesions, and 150 malignant lesions (85 ductal in situ carcinomas; 65 ductal infiltrating carcinomas). Statistical analysis showed a significant increase of IDFi in malignant lesions as compared to both normal breast and benign lesions. We noted higher IDFi in breast ductal carcinomas as compared to lobular lesions. Significant differences were observed between breast lesions with microcalcifications respect to lesions without calcifications. Noteworthy, we also found a positive association between IDFi and the expression of both ER and Ki67. CONCLUSION: Results of our study highlighted the possible role of fat in breast cancer progression suggesting a negative prognostic value of IDFi.
BACKGROUND: Recent studies showed a correlation between Body Mass Index and both breast cancer occurrence and progression. Nevertheless, no study reported an accurate evaluation of intra-ductal fat infiltrate. Therefore, the main aim of this study was to evaluate the putative association between intra-ductal fat infiltrate (IDFi) and breast cancer subtypes by using digital pathology. METHODS: We retrospectively collected 220 breast biopsies. Paraffin serial sections were used for haematoxylin and eosin staining and immunohistochemical evaluation of the following markers: estrogen receptor (ER), progesterone receptor (PR), Ki67 and c-erb2. Three haematoxylin and eosin sections for each paraffin block were digitalized. Digital slides were used to evaluate the areas of IDFi. Five randomized areas were evaluated for each slide. By using GraphPad software IDFi areas was correlated with a) breast cancer histotype, b) presence of microcalcifications and c) biomarkers expression. RESULTS: Breast biopsies were classified as follow: 20 normal breast, 50 benign lesions, and 150 malignant lesions (85 ductal in situ carcinomas; 65 ductal infiltrating carcinomas). Statistical analysis showed a significant increase of IDFi in malignant lesions as compared to both normal breast and benign lesions. We noted higher IDFi in breast ductal carcinomas as compared to lobular lesions. Significant differences were observed between breast lesions with microcalcifications respect to lesions without calcifications. Noteworthy, we also found a positive association between IDFi and the expression of both ER and Ki67. CONCLUSION: Results of our study highlighted the possible role of fat in breast cancer progression suggesting a negative prognostic value of IDFi.
Authors: Rita Bonfiglio; Filippo Galli; Michela Varani; Manuel Scimeca; Filippo Borri; Sara Fazi; Rosella Cicconi; Maurizio Mattei; Giuseppe Campagna; Tanja Schönberger; Ernest Raymond; Andreas Wunder; Alberto Signore; Elena Bonanno Journal: Int J Mol Sci Date: 2021-02-18 Impact factor: 5.923