Donghui Chen1, Li Ma2, Tianyong Hu2, Jiangqi Liu2, Baohui Chen2, Pingchang Yang3, Zhiqiang Liu4. 1. Children's Hospital Affiliated to Zhengzhou University, Zhengzhou, 457000, China. 2. Longgang ENT Hospital, Institute of ENT, Shenzhen Key Laboratory of ENT, No. 3004, Longgang Avenue, Longgang District, Shenzhen, 518172, China. 3. State Key Laboratory of Respiratory Disease for Allergy, Shenzhen University School of Medicine, Shenzhen University, Shenzhen, 518061, China. 4. Longgang ENT Hospital, Institute of ENT, Shenzhen Key Laboratory of ENT, No. 3004, Longgang Avenue, Longgang District, Shenzhen, 518172, China. liuzhiqiang05312438@126.com.
Abstract
BACKGROUND/AIMS: The etiology of inflammatory bowel disease is multifactorial and still obscure. The protective role of ubiquitin E3 ligase A20 (A20) in colitis needs to be further elucidated. This study aimed to investigate whether A20 exogenous administration restored impaired intestinal permeability and inhibited T helper (Th)2 response in mice with colitis. METHODS: The effect of A20 overexpression in colonic mucosa on epithelial barrier function and T cell differentiation was evaluated in mice with dextran sulfate sodium (DSS)-induced chronic colitis. RESULTS: A20 rectal treatment alleviated DSS-induced chronic colitis and restored impaired intestinal permeability. Oral challenge with 2% DSS elicited a Th2-type response in mice with colitis, and A20 rectal treatment inhibited CD4+ interleukin (IL)-4+ T cell differentiation and proliferation. In addition, the RNA expressions of Th2-related costimulatory molecular T-cell immunoglobulin and mucin domain (TIM)-1 and IL-4 were suppressed, while thrombospondin (TSP)-1 and interferon (IFN)-γ expressions were upregulated, after A20 rectal administration. CONCLUSION: A20 rectal treatment restores impaired intestinal permeability and inhibits activated Th2 cell response in mice with colitis.
BACKGROUND/AIMS: The etiology of inflammatory bowel disease is multifactorial and still obscure. The protective role of ubiquitin E3 ligase A20 (A20) in colitis needs to be further elucidated. This study aimed to investigate whether A20 exogenous administration restored impaired intestinal permeability and inhibited T helper (Th)2 response in mice with colitis. METHODS: The effect of A20 overexpression in colonic mucosa on epithelial barrier function and T cell differentiation was evaluated in mice with dextran sulfate sodium (DSS)-induced chronic colitis. RESULTS:A20 rectal treatment alleviated DSS-induced chronic colitis and restored impaired intestinal permeability. Oral challenge with 2% DSS elicited a Th2-type response in mice with colitis, and A20 rectal treatment inhibited CD4+ interleukin (IL)-4+ T cell differentiation and proliferation. In addition, the RNA expressions of Th2-related costimulatory molecular T-cell immunoglobulin and mucin domain (TIM)-1 and IL-4 were suppressed, while thrombospondin (TSP)-1 and interferon (IFN)-γ expressions were upregulated, after A20 rectal administration. CONCLUSION:A20 rectal treatment restores impaired intestinal permeability and inhibits activated Th2 cell response in mice with colitis.
Authors: Frank Heller; Peter Florian; Christian Bojarski; Jan Richter; Melanie Christ; Bernd Hillenbrand; Joachim Mankertz; Alfred H Gitter; Nataly Bürgel; Michael Fromm; Martin Zeitz; Ivan Fuss; Warren Strober; Jörg D Schulzke Journal: Gastroenterology Date: 2005-08 Impact factor: 22.682
Authors: Marguerite S Buzza; Tierra A Johnson; Gregory D Conway; Erik W Martin; Subhradip Mukhopadhyay; Terez Shea-Donohue; Toni M Antalis Journal: J Biol Chem Date: 2017-05-10 Impact factor: 5.157
Authors: Lars Vereecke; Mozes Sze; Conor Mc Guire; Brecht Rogiers; Yuanyuan Chu; Marc Schmidt-Supprian; Manolis Pasparakis; Rudi Beyaert; Geert van Loo Journal: J Exp Med Date: 2010-06-07 Impact factor: 14.307