Jian Li1, Yi-Ru Lu2, I-Fan Lin3, Wenyi Kang4, Hong-Bin Chen5, Hsu-Feng Lu6, Hui-Min David Wang1,5,7,8,9. 1. College of Food and Biological Engineering, Jimei University, Xiamen, China. 2. Department of Bachelor Program of Biotechnology, National Chung Hsing University, Taichung City, Taiwan. 3. Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan. 4. Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Henan University, Kaifeng, China. 5. College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, China. 6. Department of Clinical Pathology, Cheng Hsin General Hospital, Taipei, Taiwan. 7. Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung City, Taiwan. 8. College of Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan. 9. Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung City, Taiwan.
Abstract
BACKGROUND: Hibiscus sabdariffa is commonly used in daily life and its extract is applied widely in food and cosmetics. However, it has not been evaluated for its anti-aging effects. RESULTS: Hibiscus sabdariffa calyx aqueous extract (HSCAE) has shown potential collagenase activity suppression effects, together with tyrosinase activity inhibition, and anti-oxidation as a free radical scavenger. The current investigation demonstrated that HSCAE was not cytotoxic in skin fibroblasts, and it significantly decreased ultraviolet B (UVB)-induced reactive oxygen species (ROS) on a flow cytometry assay. Moreover, HSCAE reduced matrix metalloproteinase (MMP) expression, increased tissue inhibition of metalloproteinase (TIMP)-1 level, and enhanced collagen content by inhibiting collagenase activity. It also blocked mRNA and protein expressions of melanin production pathway key factors, including the microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP-1), and dopachrome tautomerase-2 (TRP-2). CONCLUSION: These results demonstrated, for the first time, the potential of HSCAE as a natural antioxidant with the ability to maintain collagen production and to decrease melanin syntheses under UVB radiation, for anti-aging effects.
BACKGROUND:Hibiscus sabdariffa is commonly used in daily life and its extract is applied widely in food and cosmetics. However, it has not been evaluated for its anti-aging effects. RESULTS:Hibiscus sabdariffa calyx aqueous extract (HSCAE) has shown potential collagenase activity suppression effects, together with tyrosinase activity inhibition, and anti-oxidation as a free radical scavenger. The current investigation demonstrated that HSCAE was not cytotoxic in skin fibroblasts, and it significantly decreased ultraviolet B (UVB)-induced reactive oxygen species (ROS) on a flow cytometry assay. Moreover, HSCAE reduced matrix metalloproteinase (MMP) expression, increased tissue inhibition of metalloproteinase (TIMP)-1 level, and enhanced collagen content by inhibiting collagenase activity. It also blocked mRNA and protein expressions of melanin production pathway key factors, including the microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP-1), and dopachrome tautomerase-2 (TRP-2). CONCLUSION: These results demonstrated, for the first time, the potential of HSCAE as a natural antioxidant with the ability to maintain collagen production and to decrease melanin syntheses under UVB radiation, for anti-aging effects.