Bruno Oliveira de Veras1, Maria Betânia Melo de Oliveira2, Fernanda Granja da Silva Oliveira3, Yago Queiroz Dos Santos4, João Ricardhis Saturnino de Oliveira2, Vera Lúcia de Menezes Lima2, Jackson Roberto Guedes da Silva Almeida3, Daniela Maria do Amaral Ferraz Navarro5, Júlio César Ribeiro de Oliveira Farias de Aguiar5, Jaciana Dos Santos Aguiar6, Krystyna Gorlach-Lira7, Caio Rodrigo Dias de Assis2, Márcia Vanusa da Silva2, Ana Catarina de Souza Lopes8. 1. Post-graduation in Tropical Medicine, Laboratory of Microbiology, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil. Electronic address: brunooveras@hotmail.com. 2. Department of Biochemistry, Laboratory of Natural Products, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil. 3. Post-graduation in Biotechnology, Laboratory of Natural Products, Federal University of São Francisco Valley, 56304-917, Pernambuco, Brazil. 4. Department of Biochemistry, Laboratory of Proteins and Bioactive Peptides, Federal University of Rio Grande do Norte, 59078-970, Natal, Rio Grande do Norte, Brazil. 5. Department of Chemistry, Center for Exact and Nature Sciences, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil. 6. Post-graduation in Biotechnology, Laboratory of Natural Products, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil. 7. Department of Molecular and Cellular Biology, Laboratory of Biology Molecular of Microorganisms, Federal University of Paraiba, 58059-900, João Pessoa, Paraiba, Brazil. 8. Post-graduation in Tropical Medicine, Laboratory of Microbiology, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Hymenaea cangaceira Pinto, Mansano & Azevedo (Fabaceae) is a Brazilian medicinal plant widely known as "Jatobá". In folk medicine, it is used to treat infections, respiratory problems, rheumatism, antitumoral, inflammation and pain, however, no activity has been scientifically validated. AIM OF THE STUDY: This study investigated chemical composition of essential oil from Hymenaea cangaceira (EOHc), antimicrobial, antinociceptive and antioxidant activities besides protection against DNA damage and hemolysis. MATERIAL AND METHODS: The essential oil was obtained by hydrodistillation, and characterized by GC-MS and GC-FID. The evaluation of antimicrobial activity was performed by microdilution method. The evaluation of the antioxidant activity was performed using the radicals DPPH, ABTS, O2- and OH-, and the protection of DNA damage using plasmid pBR322. Different experimental models were used to evaluate the antinociceptive effect (acetic acid and formalin), and evaluate the mechanisms of action involved with pharmacological antagonists (naloxone, atropine and gibenclamide) in mice. The essential oil was evaluated for hemolysis on human erythrocytes. RESULTS: The extraction of EOHc showed a yield of 0.18% on a dry basis, presenting high content of hydrocarbon sesquiterpenes (79.04%), high antioxidant activity and protect DNA from damage, besides presenting antifungal and antibacterial activity against Gram-positive and Gram-negative bacteria in vitro. It was found that the essential oil had no acute toxicity in mice up to 5000 mg/kg oral administration (o.a.), in addition to no hemolysis on human erythrocytes. The reduction of antinociceptive activity was 75%, with the opioid system as the mechanism of action. CONCLUSION: Our results validate the main activities by the traditional use attributed to H. cangaceira for antimicrobial and analgesic activity. In addition, the oil has a potent antioxidant activity, protecting the body against oxidative stress damage, adding new value to an endemic species not known to the industry.
ETHNOPHARMACOLOGICAL RELEVANCE: Hymenaea cangaceira Pinto, Mansano & Azevedo (Fabaceae) is a Brazilian medicinal plant widely known as "Jatobá". In folk medicine, it is used to treat infections, respiratory problems, rheumatism, antitumoral, inflammation and pain, however, no activity has been scientifically validated. AIM OF THE STUDY: This study investigated chemical composition of essential oil from Hymenaea cangaceira (EOHc), antimicrobial, antinociceptive and antioxidant activities besides protection against DNA damage and hemolysis. MATERIAL AND METHODS: The essential oil was obtained by hydrodistillation, and characterized by GC-MS and GC-FID. The evaluation of antimicrobial activity was performed by microdilution method. The evaluation of the antioxidant activity was performed using the radicals DPPH, ABTS, O2- and OH-, and the protection of DNA damage using plasmid pBR322. Different experimental models were used to evaluate the antinociceptive effect (acetic acid and formalin), and evaluate the mechanisms of action involved with pharmacological antagonists (naloxone, atropine and gibenclamide) in mice. The essential oil was evaluated for hemolysis on human erythrocytes. RESULTS: The extraction of EOHc showed a yield of 0.18% on a dry basis, presenting high content of hydrocarbon sesquiterpenes (79.04%), high antioxidant activity and protect DNA from damage, besides presenting antifungal and antibacterial activity against Gram-positive and Gram-negative bacteria in vitro. It was found that the essential oil had no acute toxicity in mice up to 5000 mg/kg oral administration (o.a.), in addition to no hemolysis on human erythrocytes. The reduction of antinociceptive activity was 75%, with the opioid system as the mechanism of action. CONCLUSION: Our results validate the main activities by the traditional use attributed to H. cangaceira for antimicrobial and analgesic activity. In addition, the oil has a potent antioxidant activity, protecting the body against oxidative stress damage, adding new value to an endemic species not known to the industry.