A Trullo1, J Dufourt1, M Lagha1. 1. Institut de Génétique Moléculaire de Montpellier, University of Montpellier, CNRS-UMR 5535, 1919 Route de Mende, Montpellier 34293 cedex 5, France.
Abstract
MOTIVATION: During development, progenitor cells undergo multiple rounds of cellular divisions during which transcriptional programs must be faithfully propagated. Investigating the timing of transcriptional activation, which is a highly stochastic phenomenon, requires the analysis of large amounts of data. In order to perform automatic image analysis of transcriptional activation, we developed a software that segments and tracks both small and large objects, leading the user from raw data up to the results in their final form. RESULTS: MitoTrack is a user-friendly open-access integrated software that performs the specific dual task of reporting the precise timing of transcriptional activation while keeping lineage tree history for each nucleus of a living developing embryo. The software works automatically but provides the possibility to easily supervise, correct and validate each step. AVAILABILITY AND IMPLEMENTATION: MitoTrack is an open source Python software, embedded within a graphical user interface (download here). SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
MOTIVATION: During development, progenitor cells undergo multiple rounds of cellular divisions during which transcriptional programs must be faithfully propagated. Investigating the timing of transcriptional activation, which is a highly stochastic phenomenon, requires the analysis of large amounts of data. In order to perform automatic image analysis of transcriptional activation, we developed a software that segments and tracks both small and large objects, leading the user from raw data up to the results in their final form. RESULTS: MitoTrack is a user-friendly open-access integrated software that performs the specific dual task of reporting the precise timing of transcriptional activation while keeping lineage tree history for each nucleus of a living developing embryo. The software works automatically but provides the possibility to easily supervise, correct and validate each step. AVAILABILITY AND IMPLEMENTATION: MitoTrack is an open source Python software, embedded within a graphical user interface (download here). SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
Authors: Paul Hernández-Herrera; Yamel Ugartechea-Chirino; Héctor H Torres-Martínez; Alejandro V Arzola; José Eduardo Chairez-Veloz; Berenice García-Ponce; María de la Paz Sánchez; Adriana Garay-Arroyo; Elena R Álvarez-Buylla; Joseph G Dubrovsky; Gabriel Corkidi Journal: Plant Physiol Date: 2022-02-04 Impact factor: 8.340