Ana Soriano-Lerma1,2, Antonio Magán-Fernández3, Juan Gijón3, Elena Sánchez-Fernández4, Miguel Soriano5,6, José A García-Salcedo2,5, Francisco Mesa3. 1. Department of Physiology (Faculty of Pharmacy, Campus Universitario de Cartuja), Institute of Nutrition and Food Technology "José Mataix", University of Granada, Granada, Spain. 2. Microbiology Unit, Biosanitary Research Institute ibs.GRANADA, University Hospital Virgen de las Nieves, Granada, Spain. 3. Department of Periodontics, School of Dentistry, University of Granada, Granada, Spain. 4. Department of Oral Surgery and Implant Dentistry, School of Dentistry, University of Granada, Granada, Spain. 5. GENYO, Centre for Genomics and Oncological Research: Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, Granada, Spain. 6. Center for Intensive Mediterranean Agrosystems and Agri-food Biotechnology (CIAMBITAL), University of Almeria, Almería, Spain.
Abstract
BACKGROUND: The aim of our study was to evaluate the effects of a hyaluronic acid (HA) gel at 45 days on the microbiome of implants with peri-implantitis with at least 1 year of loading. METHODS: A randomized controlled trial was conducted in peri-implantitis patients. Swabs containing the samples were collected both at baseline and after 45 days of treatment. 16S rRNA sequencing techniques were used to investigate the effect of HA gel on the subgingival microbiome. RESULTS: One hundred and eight samples of 54 patients were analyzed at baseline and after follow-up at 45 days. Three strata with different microbial composition were obtained in the samples at baseline, representing three main microbial consortia associated with peri-implantitis. Stratum 1 did not show any difference for any variable after treatment with HA, whereas in stratum 2, Streptococcus, Veillonella, Rothia, and Granulicatella did decrease (P < 0.05). Similarly, Prevotella and Campylobacter (P < 0.05) decreased in stratum 3 after treatment with HA. Microbial diversity was found to be decreased in stratum 3 (P < 0.05) after treatment with HA compared with the control group, in which an increase was found (P < 0.05). CONCLUSIONS: HA reduced the relative abundance of peri-implantitis-related microorganisms, especially the early colonizing bacteria, suggesting a specific action during the first stages in the development of the disease. HA did not alter relative abundances of non-oral genera. The use of HA in advanced stages of peri-implantitis resulted in a decrease in microbial alpha diversity, suggesting a protective action of the peri-implant site against bacteria colonization.
RCT Entities:
BACKGROUND: The aim of our study was to evaluate the effects of a hyaluronic acid (HA) gel at 45 days on the microbiome of implants with peri-implantitis with at least 1 year of loading. METHODS: A randomized controlled trial was conducted in peri-implantitis patients. Swabs containing the samples were collected both at baseline and after 45 days of treatment. 16S rRNA sequencing techniques were used to investigate the effect of HA gel on the subgingival microbiome. RESULTS: One hundred and eight samples of 54 patients were analyzed at baseline and after follow-up at 45 days. Three strata with different microbial composition were obtained in the samples at baseline, representing three main microbial consortia associated with peri-implantitis. Stratum 1 did not show any difference for any variable after treatment with HA, whereas in stratum 2, Streptococcus, Veillonella, Rothia, and Granulicatella did decrease (P < 0.05). Similarly, Prevotella and Campylobacter (P < 0.05) decreased in stratum 3 after treatment with HA. Microbial diversity was found to be decreased in stratum 3 (P < 0.05) after treatment with HA compared with the control group, in which an increase was found (P < 0.05). CONCLUSIONS: HA reduced the relative abundance of peri-implantitis-related microorganisms, especially the early colonizing bacteria, suggesting a specific action during the first stages in the development of the disease. HA did not alter relative abundances of non-oral genera. The use of HA in advanced stages of peri-implantitis resulted in a decrease in microbial alpha diversity, suggesting a protective action of the peri-implant site against bacteria colonization.
Authors: José Antonio García-Salcedo; Inmaculada López-Aliaga; Ana Soriano-Lerma; María García-Burgos; María J M Alférez; Virginia Pérez-Carrasco; Victoria Sanchez-Martin; Ángel Linde-Rodríguez; Matilde Ortiz-González; Miguel Soriano Journal: Eur J Nutr Date: 2021-08-12 Impact factor: 5.614
Authors: Elena Sánchez-Fernández; Antonio Magán-Fernández; Francisco O'Valle; Manuel Bravo; Francisco Mesa Journal: J Periodontal Implant Sci Date: 2021-02 Impact factor: 2.614
Authors: David T Wu; Jose G Munguia-Lopez; Ye Won Cho; Xiaolu Ma; Vivian Song; Zhiyue Zhu; Simon D Tran Journal: Molecules Date: 2021-11-22 Impact factor: 4.411
Authors: Ana Soriano-Lerma; Virginia Pérez-Carrasco; Manuel Sánchez-Marañón; Matilde Ortiz-González; Victoria Sánchez-Martín; Juan Gijón; José María Navarro-Mari; José Antonio García-Salcedo; Miguel Soriano Journal: Sci Rep Date: 2020-08-12 Impact factor: 4.379