Liping Song1, Xia Li2, Feng Zu3, Changbin Gao1, Bincai Wang1, Chufa Lin1, Jinxing Tu4, Aihua Wang5, Guolin Zhou6. 1. Wuhan Vegetable Research Institute, Wuhan Academy of Agriculture Science and Technology, Wuhan, 430345, China. 2. Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, People's Republic of China. 3. Industrial Crops Institute, Yunnan Academy of Agricultural Sciences, Kunming, People's Republic of China. 4. National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan, 430070, China. 5. Wuhan Vegetable Research Institute, Wuhan Academy of Agriculture Science and Technology, Wuhan, 430345, China. wangaihualt@163.com. 6. Wuhan Vegetable Research Institute, Wuhan Academy of Agriculture Science and Technology, Wuhan, 430345, China. glzhou@126.com.
Abstract
BACKGROUND: WS24-3A is a newly bred non-heading Chinese cabbage genic male-sterile line, in which sterility is controlled by a recessive gene, designated as Bra2ms. WS24-3A has been used for hybrid breeding. OBJECTIVE: To reveal the underlying molecular mechanisms responsible for the sterility of WS24-3A. METHODS: Cytological observation of the process of sterile/fertile anther development was performed to determine the tissue and stage in which sterility occurs. Phenotyping and transcriptomic analyses were performed to identify differentially expressed genes (DEGs) between sterile and fertile flower buds at different stages. RESULTS: Cytological analysis revealed no tetrads at stage 7 or at later stages of anther development, and the degradation of callose was delayed. Abnormal meiocytes were surrounded by sustaining callose that degenerated gradually in WS24-3A. Comparative transcript profiling identified 3282 DEGs during three anther developmental stages, namely, pre-meiotic anther, meiotic anther, and anthers with single-celled pollen stage. The difference in DEG percentage between up-regulated and down-regulated at meiotic anther stage was obviously larger than at the other two stages; further, most DEGs are important for male meiosis, callose synthesis and dissolution, and tapetum development. Ten DEGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. CONCLUSION: Bra2ms affected gene expression in meiocytes and associated with callose synthesis, degradation and tapetum development. Our results provide clues to elucidate the molecular mechanism of genic male sterility in non-heading Chinese cabbage.
BACKGROUND: WS24-3A is a newly bred non-heading Chinese cabbage genic male-sterile line, in which sterility is controlled by a recessive gene, designated as Bra2ms. WS24-3A has been used for hybrid breeding. OBJECTIVE: To reveal the underlying molecular mechanisms responsible for the sterility of WS24-3A. METHODS: Cytological observation of the process of sterile/fertile anther development was performed to determine the tissue and stage in which sterility occurs. Phenotyping and transcriptomic analyses were performed to identify differentially expressed genes (DEGs) between sterile and fertile flower buds at different stages. RESULTS: Cytological analysis revealed no tetrads at stage 7 or at later stages of anther development, and the degradation of callose was delayed. Abnormal meiocytes were surrounded by sustaining callose that degenerated gradually in WS24-3A. Comparative transcript profiling identified 3282 DEGs during three anther developmental stages, namely, pre-meiotic anther, meiotic anther, and anthers with single-celled pollen stage. The difference in DEG percentage between up-regulated and down-regulated at meiotic anther stage was obviously larger than at the other two stages; further, most DEGs are important for male meiosis, callose synthesis and dissolution, and tapetum development. Ten DEGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. CONCLUSION: Bra2ms affected gene expression in meiocytes and associated with callose synthesis, degradation and tapetum development. Our results provide clues to elucidate the molecular mechanism of genic male sterility in non-heading Chinese cabbage.
Entities:
Keywords:
Differentially expressed genes; Genic-male sterility; Non-heading Chinese cabbage
Authors: James D Higgins; Julien Vignard; Raphael Mercier; Alice G Pugh; F Chris H Franklin; Gareth H Jones Journal: Plant J Date: 2008-07 Impact factor: 6.417
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