Literature DB >> 3157597

Chromatofocusing coupled with automated assay for beta-hexosaminidase isoenzymes in GM2 gangliosidosis.

A Orlacchio, C Maffei, C Emiliani, G V Coppa, L Felici.   

Abstract

Good separations of the two major beta-hexosaminidase forms from human leukocytes were achieved by chromatofocusing, a technique which separates proteins on the basis of their isoelectric points. The use of an automated and reliable method is described for the identification of homozygotes and carriers of the GM2 gangliosidosis.

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Year:  1985        PMID： 3157597     DOI： 10.1007/bf01966184

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  12 in total

1.  Variation of beta-N-acetylhexosaminidase-pattern in Tay-Sachs disease.

Authors:  K Sandhoff
Journal:  FEBS Lett       Date:  1969-08       Impact factor: 4.124

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Isolation and characterization of the major beta-N-acetyl-D-glucosaminidase from human plasma.

Authors:  J A Verpoorte
Journal:  Biochemistry       Date:  1974-02-12       Impact factor: 3.162

4.  Studies on the glycosidases of jack bean meal. 3. Crystallization and properties of beta-N-acetylhexosaminidase.

Authors:  S C Li; Y T Li
Journal:  J Biol Chem       Date:  1970-10-10       Impact factor: 5.157

5.  Detection of Tay-Sachs disease heterozygotes by assay of hexosaminidase A in serum and leukocytes.

Authors:  Y Suzuki; P H Berman; K Suzuki
Journal:  J Pediatr       Date:  1971-04       Impact factor: 4.406

6.  Diagnosis of gaucher's disease and niemann-pick disease with small samples of venous blood.

Authors:  J P Kampine; R O Brady; J N Kanfer; M Feld; D Shapiro
Journal:  Science       Date:  1967-01-06       Impact factor: 47.728

7.  N-Acetyl-beta-glucosaminidases in human spleen.

Authors:  D Robinson; J L Stirling
Journal:  Biochem J       Date:  1968-04       Impact factor: 3.857

8.  The effect of membrane phospholipid acyl-chain composition on the activity of brain-beta-N-acetyl-D-glucosaminidase.

Authors:  A Orlacchio; C Maffei; L Binaglia; G Porcellati
Journal:  Biochem J       Date:  1981-05-01       Impact factor: 3.857

9.  Cleavage of the (1 goes to 3)-2-acetamido-2-deoxy-beta-D-glucopyranosyl linkage present in keratan sulfate. The A and B isoenzymes of human liver hexosaminidase (EC 3.2.1.30).

Authors:  S Toma; G Coppa; P V Donnelly; R Ricci; N Di Ferrante; S K Srivastava
Journal:  Carbohydr Res       Date:  1981-10-16       Impact factor: 2.104

10.  Tay-Sachs disease: prenatal diagnosis.

Authors:  J S O'Brien; S Okada; D L Fillerup; M L Veath; B Adornato; P H Brenner; J G Leroy
Journal:  Science       Date:  1971-04-02       Impact factor: 47.728
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