Literature DB >> 3156960

Expression of a gene for glucan-binding protein from Streptococcus mutans in Escherichia coli.

R R Russell, D Coleman, G Dougan.   

Abstract

The structural gene for a glucan-binding protein (GBP) of Streptococcus mutans has been inserted into a bacteriophage lambda vector and expressed in Escherichia coli K12. Lysates of E. coli infected with the recombinant phage contain an antigenic protein of the same size as S. mutans GBP. The GBP synthesized in E. coli can be affinity-purified on immobilized glucan and antiserum raised against it has been shown to precipitate fructosyltransferase activity from S. mutans.

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Year:  1985        PMID： 3156960     DOI： 10.1099/00221287-131-2-295

Source DB:  PubMed          Journal:  J Gen Microbiol        ISSN： 0022-1287


  24 in total

1.  Linkage of sucrose-metabolizing genes in Streptococcus mutans.

Authors:  D Perry; H K Kuramitsu
Journal:  Infect Immun       Date:  1990-10       Impact factor: 3.441

2.  Nucleotide sequence of a glucosyltransferase gene from Streptococcus sobrinus MFe28.

Authors:  J J Ferretti; M L Gilpin; R R Russell
Journal:  J Bacteriol       Date:  1987-09       Impact factor: 3.490

3.  Isolation of a glucan-binding domain of glucosyltransferase (1,6-alpha-glucan synthase) from Streptococcus sobrinus.

Authors:  G Mooser; C Wong
Journal:  Infect Immun       Date:  1988-04       Impact factor: 3.441

4.  Cloning and expression of two Streptococcus mutans glucosyltransferases in Escherichia coli K-12.

Authors:  M L Gilpin; R R Russell; P Morrissey
Journal:  Infect Immun       Date:  1985-08       Impact factor: 3.441

5.  Recent advances in defining the cariogenicity of mutans streptococci: molecular genetic approaches.

Authors:  H K Kuramitsu
Journal:  Eur J Epidemiol       Date:  1987-09       Impact factor: 8.082

6.  Isolation of DNA encoding sucrase genes from Streptococcus salivarius and partial characterization of the enzymes expressed in Escherichia coli.

Authors:  C M Houck; J R Pear; R Elliott; J T Perchorowicz
Journal:  J Bacteriol       Date:  1987-08       Impact factor: 3.490

7.  Overproduction of a dextranase inhibitor by Streptococcus sobrinus mutants.

Authors:  S Y Wanda; A Camilli; H M Murchison; R Curtiss
Journal:  J Bacteriol       Date:  1994-12       Impact factor: 3.490

8.  Virulence of protein A-deficient and alpha-toxin-deficient mutants of Staphylococcus aureus isolated by allele replacement.

Authors:  A H Patel; P Nowlan; E D Weavers; T Foster
Journal:  Infect Immun       Date:  1987-12       Impact factor: 3.441

9.  Multiple glucan-binding proteins of Streptococcus sobrinus.

Authors:  Y Ma; M O Lassiter; J A Banas; M Y Galperín; K G Taylor; R J Doyle
Journal:  J Bacteriol       Date:  1996-03       Impact factor: 3.490

10.  Glucans synthesized in situ in experimental salivary pellicle function as specific binding sites for Streptococcus mutans.

Authors:  K M Schilling; W H Bowen
Journal:  Infect Immun       Date:  1992-01       Impact factor: 3.441
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