Physical, mechanical and in vitro biological evaluation of synthesized biosurfactant-modified silanated-gelatin/sodium alginate/45S5 bioglass bone tissue engineering scaffolds.

The aim of this study is to fabricate a highly porous scaffold based on gelatin/sodium alginate/45S5 bioglass with improved mechanical strength. Glycidoxypropyltrimethoxysilane (GPTMS) is used as a cross-linker and for the first time a nonionic bio-surfactant (Tween 80) is introduced to the composition to evaluate whether the essential properties of a suitable scaffold for bone substitution can be reached or not. The composite scaffolds are prepared through freeze-drying of suspension containing various ratios of gelatin/sodium alginate/45S5 bioglass. Characterization of fabricated scaffolds is carried out. SEM micrographs reveal that all samples are highly porous however incorporation of 1% v/v tween 80 results in well-shaped pores, with sizes ranging between 100 and 200 µm which is appropriate for tissue regeneration. Compressive strength of foamed scaffolds in contact with body solution has been enhanced from 0.37 to 1.41 MPa due to addition of tween 80. Foamed scaffold reinforced with tween 80 maintained its structural stability within 7 days immersion in simulated body fluid (SBF) despite the absorption of water 15 times its weight. Moreover, in vitro calcium phosphate precipitation is well observed on the surface of scaffolds.