Marzia Vasarri1, Manuela Leri1, Emanuela Barletta1, Matteo Ramazzotti1, Riccardo Marzocchini1, Donatella Degl'Innocenti2. 1. Dipartimento di Scienze Biomediche, Sperimentali e Cliniche "Mario Serio", Università degli Studi di Firenze, viale Morgagni 50, 50134, Firenze, Italy. 2. Dipartimento di Scienze Biomediche, Sperimentali e Cliniche "Mario Serio", Università degli Studi di Firenze, viale Morgagni 50, 50134, Firenze, Italy; Centro Interuniversitario di Biologia Marina ed Ecologia Applicata "G. Bacci", Viale N. Sauro, 4, 57128, Livorno, Italy. Electronic address: donatella.deglinnocenti@unifi.it.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Posidonia oceanica (L.) Delile is an endemic seagrass of the Mediterranean Sea whose use has been documented as a traditional herbal remedy for diabetes and hypertension. Our recently described Posidonia oceanica leaves extract is a phytocomplex endowed with interesting bioactivities, including the inibitory property on human cancer cell migration. AIM OF THE STUDY: The aim of this study was to investigate the anti-inflammatory effects of P. oceanica extract underlying its mechanism of action. MATERIALS AND METHODS: We explored the anti-inflammatory effects of P. oceanica extract on RAW264.7 murine macrophages activated by LPS. We investigated the reactive oxygen species (ROS) and nitric oxide (NO) production and the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Then, we examined P. oceanica extract role on the regulation of NF-κB signaling pathway. RESULTS: P. oceanica phytocomplex exhibited a strong ability to inhibit oxidative stress by affecting the production of both ROS and NO and to reduce iNOS and COX-2 levels. In addition, it was evidenced its anti-inflammatory role via inhibiting NF-κB signaling pathway through modulation of ERK1/2 and Akt intracellular cascades. CONCLUSIONS: Our results recognize an anti-inflammatory role of P. oceanica phytocomplex particularly emphasizing its cell safe mechanism of action. In conclusion, the marine plant P. oceanica may be of great interest for scientific research as a source of promising molecules for designing alternative strategies to the conventional treatment of inflammatory diseases.
ETHNOPHARMACOLOGICAL RELEVANCE: Posidonia oceanica (L.) Delile is an endemic seagrass of the Mediterranean Sea whose use has been documented as a traditional herbal remedy for diabetes and hypertension. Our recently described Posidonia oceanica leaves extract is a phytocomplex endowed with interesting bioactivities, including the inibitory property on humancancer cell migration. AIM OF THE STUDY: The aim of this study was to investigate the anti-inflammatory effects of P. oceanica extract underlying its mechanism of action. MATERIALS AND METHODS: We explored the anti-inflammatory effects of P. oceanica extract on RAW264.7murine macrophages activated by LPS. We investigated the reactive oxygen species (ROS) and nitric oxide (NO) production and the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Then, we examined P. oceanica extract role on the regulation of NF-κB signaling pathway. RESULTS:P. oceanica phytocomplex exhibited a strong ability to inhibit oxidative stress by affecting the production of both ROS and NO and to reduce iNOS and COX-2 levels. In addition, it was evidenced its anti-inflammatory role via inhibiting NF-κB signaling pathway through modulation of ERK1/2 and Akt intracellular cascades. CONCLUSIONS: Our results recognize an anti-inflammatory role of P. oceanica phytocomplex particularly emphasizing its cell safe mechanism of action. In conclusion, the marine plant P. oceanica may be of great interest for scientific research as a source of promising molecules for designing alternative strategies to the conventional treatment of inflammatory diseases.
Authors: Laura Micheli; Marzia Vasarri; Emanuela Barletta; Elena Lucarini; Carla Ghelardini; Donatella Degl'Innocenti; Lorenzo Di Cesare Mannelli Journal: Mar Drugs Date: 2021-01-21 Impact factor: 5.118
Authors: Marzia Vasarri; Anna Maria De Biasi; Emanuela Barletta; Carlo Pretti; Donatella Degl'Innocenti Journal: Mar Drugs Date: 2021-08-25 Impact factor: 5.118