| Literature DB >> 3156034 |
O Bachs, K S Famulski, F Mirabelli, E Carafoli.
Abstract
Three plasma membrane subfractions have been isolated and characterized from rat liver cells. The high affinity Ca2+-stimulated ATPase is highly enriched in the bile canalicular subfraction. Taking into account cross-contamination by the blood sinusoidal and lateral membranes it is suggested that the high-affinity Ca2+-ATPase is located exclusively in this fraction. The high-affinity Ca2+-ATPase is coupled to Ca2+ transport, is calmodulin-insensitive, sensitive to vanadate under appropriate experimental conditions and is strongly inhibited by La3+. In the presence of Ca2+ and ATP the ATPase forms a phosphorylated intermediate of molecular mass about 200 kDa.Entities:
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Year: 1985 PMID： 3156034 DOI： 10.1111/j.1432-1033.1985.tb08710.x
Source DB: PubMed Journal: Eur J Biochem ISSN： 0014-2956