| Literature DB >> 3155864 |
P Januszewicz, J Gutkowska, A De Lean, G Thibault, R Garcia, J Genest, M Cantin.
Abstract
The synthetic fragment (Arg 101-Tyr 126) of atrial natriuretic factor (ANF) induces release of arginine vasopressin from the isolated posterior lobe of the rat hypophysis in vitro. At a physiological concentration (3 X 10(-10)M) ANF was three times more effective than 61 mM KCL. In vitro binding studies with 125I-ANF revealed the presence of high affinity receptor sites displaying a pK = 9.9, a Kd = 0.14 nM, a Bmax = 20 fmol/posterior lobe and and IC50 = 200 pM. These results suggest that arginine vasopressin release by synthetic atrial natriuretic factor may be receptor mediated.Entities:
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Year: 1985 PMID： 3155864 DOI： 10.3181/00379727-178-2-rc4
Source DB: PubMed Journal: Proc Soc Exp Biol Med ISSN： 0037-9727