Literature DB >> 31554688

Authentication Analysis of MT-4 Cells Distributed by the National Institutes of Health AIDS Reagent Program.

Melissa V Fernandez1, Krista A Delviks-Frankenberry2, David A Scheiblin3, Christine Happel4, Vinay K Pathak2, Eric O Freed5.   

Abstract

The MT-4 human T-cell line expresses HTLV-1 Tax and is permissive for replication of an HIV-1 gp41 mutant lacking the cytoplasmic tail. MT-4 cells (lot 150048), distributed by the NIH AIDS Reagent Program (NIH-ARP), were found to be Tax deficient and unable to host replication of the gp41-truncated HIV-1 mutant. These findings, together with short tandem repeat profiling, established that lot 150048 are not bona fide MT-4 cells.
Copyright © 2019 American Society for Microbiology.

Entities:  

Keywords:  MT-4; STR profiling; authentication; short tandem repeat

Mesh：

Substances：

Year:  2019        PMID： 31554688      PMCID： PMC6880167          DOI： 10.1128/JVI.01390-19

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  22 in total

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2.  Cell line cross-contamination initiative: an interactive reference database of STR profiles covering common cancer cell lines.

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Journal:  J Biomol Tech       Date:  2018-05-10

4.  A simple assay based on HIV infection preventing the reclustering of MT-4 cells.

Authors:  G Szucs; J L Melnick; F B Hollinger
Journal:  Bull World Health Organ       Date:  1988       Impact factor: 9.408

5.  Biochemical evidence of a role for matrix trimerization in HIV-1 envelope glycoprotein incorporation.

Authors:  Philip R Tedbury; Mariia Novikova; Sherimay D Ablan; Eric O Freed
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6.  Second-site compensatory mutations of HIV-1 capsid mutations.

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7.  Homotypic cell-cell adhesion induced by human T cell leukemia virus type 1 tax protein in T cell lines.

Authors:  Toshiyuki Takahashi; Masaya Higuchi; Masaya Fukushi; Masayasu Oie; Masaaki Ito; Masahiro Fujii
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8.  Evidence that productive human immunodeficiency virus type 1 assembly can occur in an intracellular compartment.

Authors:  Anjali Joshi; Sherimay D Ablan; Ferri Soheilian; Kunio Nagashima; Eric O Freed
Journal:  J Virol       Date:  2009-03-18       Impact factor: 5.103

9.  Evidence for a functional interaction between the V1/V2 and C4 domains of human immunodeficiency virus type 1 envelope glycoprotein gp120.

Authors:  E O Freed; M A Martin
Journal:  J Virol       Date:  1994-04       Impact factor: 5.103

10.  Comparison of cell-based assays for the identification and evaluation of competitive CXCR4 inhibitors.

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2.  Elucidating the Basis for Permissivity of the MT-4 T-Cell Line to Replication of an HIV-1 Mutant Lacking the gp41 Cytoplasmic Tail.

Authors:  Melissa V Fernandez; Huxley K Hoffman; Nairi Pezeshkian; Philip R Tedbury; Schuyler B van Engelenburg; Eric O Freed
Journal:  J Virol       Date:  2020-11-09       Impact factor: 5.103

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4.  Multiplexed tat-Targeting CRISPR-Cas9 Protects T Cells from Acute HIV-1 Infection with Inhibition of Viral Escape.

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Review 5.  Molecular Action of Polyphenols in Leukaemia and Their Therapeutic Potential.

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