Kailin Yang1, Liuting Zeng2, Anqi Ge3, Zhouhua Chen4, Tingting Bao5, Zhiyong Long6, Jinwen Ge7, Lizhong Huang8. 1. Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China; Capital Medical University, Beijing, China. 2. Hunan University of Chinese Medicine, Changsha, Hunan Province, China. Electronic address: 2057188405@qq.com. 3. The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan Province, China. 4. The Second People's Hospital of Xiangtan City, Xiangtan, Hunan Province, China. 5. Beijing University of Chinese Medicine, Beijing, China. 6. Shantou University Medical College, Shantou, Guangdong Province, China. 7. Hunan University of Chinese Medicine, Changsha, Hunan Province, China. Electronic address: 40831556@qq.com. 8. Hunan University of Chinese Medicine, Changsha, Hunan Province, China. Electronic address: hlz992002@163.com.
Abstract
OBJECTIVE: To investigate the regulation mechanism of baicalin on triple negative breast cancer (TNBC)'s biological network by a systematic biological strategy and cytology experiment. METHODS: A systematic biological methodology is utilized to predict the potential targets of baicalin, collect the genes of TNBC, and analyze the TNBC and baicalin's network. After the systematic biological analysis is performed, the cytology experiment, real-time quantitative PCR (qPCR) is used to validate the key biological processes and signaling pathways. RESULTS: After systematic biological analysis, two networks were constructed and analyzed: (1) TNBC network; (2) Baicalin-TNBC protein-protein interaction (PPI) network. Several TNBC-related, treatment-related targets, clusters, signaling pathways and biological processes were found. Cytology experiment shows that baicalin can inhibit the proliferation, migration and invasion of breast cancer MDA-MB-231 cells in vitro (P < 0.05). The results of qPCR showed that baicalin increase the expression of E-cadherin mRNA, and decrease the expression of vimentin, β-catenin, c-Myc and MMP-7 mRNA in LPS-induced breast cancer MDA-MB-231 cells (P < 0.05). CONCLUSION: Baicalin may achieve anti-tumor effects through regulating the targets, biological processes and pathways found in this research.
OBJECTIVE: To investigate the regulation mechanism of baicalin on triple negative breast cancer (TNBC)'s biological network by a systematic biological strategy and cytology experiment. METHODS: A systematic biological methodology is utilized to predict the potential targets of baicalin, collect the genes of TNBC, and analyze the TNBC and baicalin's network. After the systematic biological analysis is performed, the cytology experiment, real-time quantitative PCR (qPCR) is used to validate the key biological processes and signaling pathways. RESULTS: After systematic biological analysis, two networks were constructed and analyzed: (1) TNBC network; (2) Baicalin-TNBC protein-protein interaction (PPI) network. Several TNBC-related, treatment-related targets, clusters, signaling pathways and biological processes were found. Cytology experiment shows that baicalin can inhibit the proliferation, migration and invasion of breast cancerMDA-MB-231 cells in vitro (P < 0.05). The results of qPCR showed that baicalin increase the expression of E-cadherin mRNA, and decrease the expression of vimentin, β-catenin, c-Myc and MMP-7 mRNA in LPS-induced breast cancerMDA-MB-231 cells (P < 0.05). CONCLUSION:Baicalin may achieve anti-tumor effects through regulating the targets, biological processes and pathways found in this research.