| Literature DB >> 31544993 |
Yoichi Sasaki1, Mio Oshikawa2, Pankaj Bharmoria1, Hironori Kouno1, Akiko Hayashi-Takagi3,4, Moritoshi Sato5, Itsuki Ajioka2,4, Nobuhiro Yanai1,4, Nobuo Kimizuka1.
Abstract
Photon upconversion (UC) from near-infrared (NIR) light to visible light has enabled optogenetic manipulations in deep tissues. However, materials for NIR optogenetics have been limited to inorganic UC nanoparticles. Herein, NIR-light-triggered optogenetics using biocompatible, organic TTA-UC hydrogels is reported. To achieve triplet sensitization even in highly viscous hydrogel matrices, a NIR-absorbing complex is covalently linked with energy-pooling acceptor chromophores, which significantly elongates the donor triplet lifetime. The donor and acceptor are solubilized in hydrogels formed from biocompatible Pluronic F127 micelles, and heat treatment endows the excited triplets in the hydrogel with remarkable oxygen tolerance. Combined with photoactivatable Cre recombinase technology, NIR-light stimulation successfully performs genome engineering resulting in the formation of dendritic-spine-like structures of hippocampal neurons.Entities:
Keywords: genome engineering; near-infrared light; photon upconversion; singlet-to-triplet absorption; triplet-triplet annihilation
Year: 2019 PMID： 31544993 DOI： 10.1002/anie.201911025
Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336