Bruna Pizziolo Coura1, Taynara Asevedo Campos de Resende2, Vinícius César Barbosa de Menezes2, Vanessa Fátima Bernardes1, Silvia Ferreira de Sousa2, Marina Gonçalves Diniz1, Ricardo Santiago Gomez3, Carolina Cavalieri Gomes4. 1. Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil. 2. Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil. 3. Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil. Electronic address: rsgomez@ufmg.br. 4. Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil. Electronic address: carolinacgomes@ufmg.br.
Abstract
OBJECTIVE: Driver oncogenic mutations have been reported in several benign neoplasms. While ameloblastomas show BRAF p.V600E mutations, adenomatoid odontogenic tumours harbour either KRAS p.G12R or p.G12 V. The lack of understanding of the core molecular changes involved in tumour initiation and progression represents a critical barrier to developing new strategies for cancer detection and prevention. Considering the fact that ameloblastoma and adenomatoid odontogenic tumours can originate from dental follicles, we hypothesized that the BRAF and KRAS mutations might be early events in odontogenic tumours tumourigenesis. We aimed to assess BRAF and KRAS mutations in dental follicles associated with asymptomatic impacted teeth. DESIGN: Forty-eight dental follicles containing odontogenic epithelial remnants were included in the study. As ameloblastomas most often occur in the posterior mandible and adenomatoid odontogenic tumours have a predilection for the anterior jaws, we assessed by allele-specific qPCR the presence of BRAF p.V600E in 32 dental follicles associated with impacted 3rd mandibular molar teeth and KRAS p.G12 V and KRAS p.G12R mutations in 16 dental follicle specimens obtained from around impacted anterior teeth. Sanger sequencing was used as an additional method. RESULTS: None of the dental follicle cases tested positive for the mutations. CONCLUSION: In conclusion, we tried to detect the early genetic events associated with odontogenic tumours development in dental follicles, but we were unable to showcase that BRAF p.V600E and KRAS p.G12R or p.G12 V mutations are the early genetic events associated with odontogenic tumours development.
OBJECTIVE: Driver oncogenic mutations have been reported in several benign neoplasms. While ameloblastomas show BRAFp.V600E mutations, adenomatoid odontogenic tumours harbour either KRASp.G12R or p.G12 V. The lack of understanding of the core molecular changes involved in tumour initiation and progression represents a critical barrier to developing new strategies for cancer detection and prevention. Considering the fact that ameloblastoma and adenomatoid odontogenic tumours can originate from dental follicles, we hypothesized that the BRAF and KRAS mutations might be early events in odontogenic tumours tumourigenesis. We aimed to assess BRAF and KRAS mutations in dental follicles associated with asymptomatic impacted teeth. DESIGN: Forty-eight dental follicles containing odontogenic epithelial remnants were included in the study. As ameloblastomas most often occur in the posterior mandible and adenomatoid odontogenic tumours have a predilection for the anterior jaws, we assessed by allele-specific qPCR the presence of BRAFp.V600E in 32 dental follicles associated with impacted 3rd mandibular molar teeth and KRAS p.G12 V and KRASp.G12R mutations in 16 dental follicle specimens obtained from around impacted anterior teeth. Sanger sequencing was used as an additional method. RESULTS: None of the dental follicle cases tested positive for the mutations. CONCLUSION: In conclusion, we tried to detect the early genetic events associated with odontogenic tumours development in dental follicles, but we were unable to showcase that BRAFp.V600E and KRASp.G12R or p.G12 V mutations are the early genetic events associated with odontogenic tumours development.
Authors: Lucrezia Togni; Antonio Zizzi; Roberta Mazzucchelli; Andrea Santarelli; Corrado Rubini; Marco Mascitti Journal: Int J Oral Sci Date: 2022-04-25 Impact factor: 24.897
Authors: Victor Coutinho Bastos; Jéssica Gardone Vitório; Roberta Rayra Martins-Chaves; Flávia Leite-Lima; Yuri Abner Rocha Lebron; Victor Rezende Moreira; Filipe Fideles Duarte-Andrade; Thaís Dos Santos Fontes Pereira; Lucilaine Valéria de Souza Santos; Liséte Celina Lange; Adriana Nori de Macedo; Gisele André Baptista Canuto; Carolina Cavaliéri Gomes; Ricardo Santiago Gomez Journal: Front Oral Health Date: 2021-06-18