H Yao1, J K Xu2, N Y Zheng3, J L Wang4, S W Mok5, Y W Lee6, L Shi7, J Y Wang8, J Yue9, S H Yung10, P J Hu11, Y C Ruan12, Y F Zhang13, K W Ho14, L Qin15. 1. Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, PR China. Electronic address: yaohao@link.cuhk.edu.hk. 2. Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, PR China. Electronic address: shejun1000@126.com. 3. Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, PR China. Electronic address: zhengnianye@outlook.com. 4. Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, PR China. Electronic address: wangjialicuhk@gmail.com. 5. Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, PR China. Electronic address: storymsw@link.cuhk.edu.hk. 6. Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, PR China. Electronic address: angellee@ort.cuhk.edu.hk. 7. Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, PR China. Electronic address: shiliu_gk@126.com. 8. Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, PR China. Electronic address: wangjinyu_hust@outlook.com. 9. Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, PR China. Electronic address: rjnfm3083@163.com. 10. Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, PR China. Electronic address: patrick@ort.cuhk.edu.hk. 11. Department of Biomedical Engineering, Polytechnic University of Hong Kong, Hong Kong SAR, PR China. Electronic address: pei-jie.hu@connect.polyu.hk. 12. Department of Biomedical Engineering, Polytechnic University of Hong Kong, Hong Kong SAR, PR China. Electronic address: sharon.yc.ruan@polyu.edu.hk. 13. School of Life Science and Technology, ShanghaiTech University, Shanghai, PR China. Electronic address: Zhangyifeng126@126.com. 14. Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, PR China. Electronic address: kevinho@cuhk.edu.hk. 15. Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, PR China. Electronic address: lingqin@cuhk.edu.hk.
Abstract
OBJECTIVE: To explore the effects of Mg2+ on the expression of osteoarthritic markers in human cartilage and synovium tissue explants. To investigate the therapeutic effect of intra-articular injection of Mg2+ in an established rat OA (Osteoarthritis) model of anterior cruciate ligament transection with partial medial meniscectomy (ACLT + PMM). DESIGN: Human cartilage and synovium explants were collected from total knee replacement surgeries and incubated with MgCl2 (20 mmol/L) in vitro. A rat OA model was established by ACLT + PMM surgery in 450-500 g male Sprague Dawley (SD) rats. To select the optimal dose, intra-articular injections of MgCl2 (0.05, 0.5, 5 mol/L) were performed at 4 weeks after the surgery every 3 days for 2 weeks. The effect of optimized MgCl2 was further determined by histology, immunohistochemistry, and quantitative real-time polymerase chain reaction. RESULTS: The expressions of osteoarthritic markers in human cartilage and synovium explants were inhibited by Mg2+in vitro. Immunohistochemical analysis further suggested the inhibitory effects of Mg2+ on the expression of MMP-13 and IL-6 in the human tissue explants. Cartilage degeneration and synovitis in ACLT + PMM rats were significantly improved by intra-articular injections of Mg2+ (0.5 mol/L). Immunohistochemical analysis also showed the regulatory effects of Mg2+ on osteoarthritic markers in both cartilage and synovium in rats, consistent with in vitro results. CONCLUSION: Intra-articular injections of Mg2+ at 0.5 mol/L attenuate the progression of OA in the ACLT + PMM rat model. Such effect was at least in part explained by the promotion of cartilage matrix synthesis and the suppression of synovial inflammation.
OBJECTIVE: To explore the effects of Mg2+ on the expression of osteoarthritic markers in humancartilage and synovium tissue explants. To investigate the therapeutic effect of intra-articular injection of Mg2+ in an established rat OA (Osteoarthritis) model of anterior cruciate ligament transection with partial medial meniscectomy (ACLT + PMM). DESIGN:Humancartilage and synovium explants were collected from total knee replacement surgeries and incubated with MgCl2 (20 mmol/L) in vitro. A rat OA model was established by ACLT + PMM surgery in 450-500 g male Sprague Dawley (SD) rats. To select the optimal dose, intra-articular injections of MgCl2 (0.05, 0.5, 5 mol/L) were performed at 4 weeks after the surgery every 3 days for 2 weeks. The effect of optimized MgCl2 was further determined by histology, immunohistochemistry, and quantitative real-time polymerase chain reaction. RESULTS: The expressions of osteoarthritic markers in humancartilage and synovium explants were inhibited by Mg2+in vitro. Immunohistochemical analysis further suggested the inhibitory effects of Mg2+ on the expression of MMP-13 and IL-6 in the human tissue explants. Cartilage degeneration and synovitis in ACLT + PMM rats were significantly improved by intra-articular injections of Mg2+ (0.5 mol/L). Immunohistochemical analysis also showed the regulatory effects of Mg2+ on osteoarthritic markers in both cartilage and synovium in rats, consistent with in vitro results. CONCLUSION: Intra-articular injections of Mg2+ at 0.5 mol/L attenuate the progression of OA in the ACLT + PMM rat model. Such effect was at least in part explained by the promotion of cartilage matrix synthesis and the suppression of synovial inflammation.
Authors: Ruijun Bai; Michael Z Miao; Hui Li; Yiqing Wang; Ruixue Hou; Ke He; Xuan Wu; Hongyu Jin; Chao Zeng; Yang Cui; Guanghua Lei Journal: Arthritis Res Ther Date: 2022-07-08 Impact factor: 5.606
Authors: Mikołaj Dąbrowski; Anetta Zioła-Frankowska; Marcin Frankowski; Jacek Kaczmarczyk; Łukasz Kubaszewski Journal: Int J Environ Res Public Health Date: 2021-03-22 Impact factor: 3.390