Konstantine V Kirienko1, Valentina P Apryshko2, Anna A Naumova1, Margarita A Kharitonova1, Aleksey A Klepukov1, Anton I Bolt1, Irina Y Ermilova1, Anna G Mironova3, Yana V Bozina1, Elena B Lebedeva1, Ekaterina Y Simonenko4, Gabor Vajta5, Sergey A Yakovenko6. 1. Altravita IVF Clinic, 4A Nagornaya 117186, Moscow, Russia. 2. Altravita IVF Clinic, 4A Nagornaya 117186, Moscow, Russia; Lomonosov Moscow State University, Faculty of Biology, 1/12 Leninskie gori, Moscow 119234, Russia. 3. Altravita IVF Clinic, 4A Nagornaya 117186, Moscow, Russia; Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Kosygina st., 4., Moscow 119334, Russia. 4. Lomonosov Moscow State University, Faculty of Physics, Biophysics Department, 1/2 Leninskie gori, Moscow 119991, Russia. 5. RVT Australia, 20 Slate Close, Brinsmead QLD 4870, Australia. Electronic address: gabor.vajta@hotmail.com. 6. Altravita IVF Clinic, 4A Nagornaya 117186, Moscow, Russia; Lomonosov Moscow State University, Faculty of Physics, Biophysics Department, 1/2 Leninskie gori, Moscow 119991, Russia.
Abstract
RESEARCH QUESTION: Does complete mechanical removal of the zona pellucida modify the outcome of transfer of vitrified-warmed human blastocysts? DESIGN: In a prospective randomized controlled study, 419 couples were allocated to either zona pellucida-free (n = 209) or zona intact (n = 210) vitrified-warmed embryo transfer. Main outcome measures included clinical pregnancy, implantation and ongoing pregnancy rates. RESULTS: Transfer of zona pellucida-free blastocysts resulted in clinical pregnancy, implantation and ongoing pregnancy rates (35,9%, 33,9% and 32,1%, respectively), similar to those achieved with zona intact control embryos (39%, 36,4% and 33,1%, respectively). CONCLUSION:Total mechanical removal of the zona pellucida did not affect the tested parameters of clinical outcomes.
RCT Entities:
RESEARCH QUESTION: Does complete mechanical removal of the zona pellucida modify the outcome of transfer of vitrified-warmed human blastocysts? DESIGN: In a prospective randomized controlled study, 419 couples were allocated to either zona pellucida-free (n = 209) or zona intact (n = 210) vitrified-warmed embryo transfer. Main outcome measures included clinical pregnancy, implantation and ongoing pregnancy rates. RESULTS: Transfer of zona pellucida-free blastocysts resulted in clinical pregnancy, implantation and ongoing pregnancy rates (35,9%, 33,9% and 32,1%, respectively), similar to those achieved with zona intact control embryos (39%, 36,4% and 33,1%, respectively). CONCLUSION: Total mechanical removal of the zona pellucida did not affect the tested parameters of clinical outcomes.