| Literature DB >> 31526571 |
Kutubuddin A Molla1, Yinong Yang2.
Abstract
SpCas9 creates blunt end cuts in the genome and generates random and unpredictable mutations through error-prone repair systems. However, a growing body of recent evidence points instead to Cas9-induced staggered end generation, nonrandomness of mutations, and the predictability of editing outcomes using machine learning models.Keywords: CRISPR therapeutics; Cas9 mutagenesis; Cas9 repair outcome prediction; Cas9 staggered cut; machine learning; template-free precise editing
Mesh：
Year: 2019 PMID： 31526571 DOI： 10.1016/j.tibtech.2019.08.002
Source DB: PubMed Journal: Trends Biotechnol ISSN： 0167-7799 Impact factor: 19.536