Benjamin Kelly1,2, Sheyanth Mohanakumar1, Niklas Telinius3, Mathias Alstrup1,2, Vibeke Hjortdal2. 1. Department of Cardiothoracic and Vascular Surgery, Aarhus University Hospital, Aarhus, Denmark. 2. Department of Clinical Medicine, Aarhus University, Aarhus, Denmark. 3. Department of Ophthalmology, Aarhus University Hospital, Aarhus, Denmark.
Abstract
Background: Knowledge of functional parameters that can be used for evaluation of upper extremity lymphatic function is limited. This study aims to evaluate near-infrared fluorescence (NIRF) imaging for evaluation of lymphatic function in the human arm. Methods and Results: Ten healthy male volunteers (age 25.7 ± 1.3 years) were included and examined at two occasions. The lymphatic functional frequency and velocity were examined at baseline, during hyperthermia and after exercise. In addition, the maximum pressure generated by contraction of the lymphatic vessels was estimated. The mean contraction frequency was found to be 0.9 ± 0.4/min, and the mean velocity of the propulsions was 1.1 ± 0.3 cm/s. The average maximal pressure generated by the contraction of the lymphatic vessels was 59 ± 12 mmHg. No significant difference in frequency, velocity, or pumping pressure was found between the two visits (p > 0.05). Local hyperthermia increased contraction frequency significantly, whereas exercise decreased frequency and increased propulsion velocity. Conclusions: The functional lymphatic parameters evaluated by NIRF imaging showed good repeatability with no significant difference between visits. Future examinations should be conducted with standardization of temperature and exercise, as these parameters were shown to alter lymphatic function.
Background: Knowledge of functional parameters that can be used for evaluation of upper extremity lymphatic function is limited. This study aims to evaluate near-infrared fluorescence (NIRF) imaging for evaluation of lymphatic function in the human arm. Methods and Results: Ten healthy male volunteers (age 25.7 ± 1.3 years) were included and examined at two occasions. The lymphatic functional frequency and velocity were examined at baseline, during hyperthermia and after exercise. In addition, the maximum pressure generated by contraction of the lymphatic vessels was estimated. The mean contraction frequency was found to be 0.9 ± 0.4/min, and the mean velocity of the propulsions was 1.1 ± 0.3 cm/s. The average maximal pressure generated by the contraction of the lymphatic vessels was 59 ± 12 mmHg. No significant difference in frequency, velocity, or pumping pressure was found between the two visits (p > 0.05). Local hyperthermia increased contraction frequency significantly, whereas exercise decreased frequency and increased propulsion velocity. Conclusions: The functional lymphatic parameters evaluated by NIRF imaging showed good repeatability with no significant difference between visits. Future examinations should be conducted with standardization of temperature and exercise, as these parameters were shown to alter lymphatic function.
Authors: Mathias Alstrup; Andreas L Johannessen; Sheyanth Mohanakumar; Birgitte V Offersen; Vibeke E Hjortdal Journal: Plast Reconstr Surg Glob Open Date: 2021-08-25
Authors: Sheyanth Mohanakumar; Benjamin Kelly; Aida Luiza Ribeiro Turquetto; Mathias Alstrup; Luciana Patrick Amato; Milena Schiezari Ru Barnabe; João Bruno Dias Silveira; Fernando Amaral; Paulo Henrique Manso; Marcelo Biscegli Jatene; Vibeke Elisabeth Hjortdal Journal: Physiol Rep Date: 2021-06
Authors: Andreas L Johannessen; Mathias Alstrup; Vibeke E Hjortdal; Birgitte V Offersen; Sheyanth Mohanakumar Journal: Plast Reconstr Surg Glob Open Date: 2022-09-16