Luciana Sartori1, Fábio P Sellera2, Quézia Moura3, Brenda Cardoso3, Louise Cerdeira1, Nilton Lincopan4. 1. Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil. 2. Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil. 3. Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. 4. Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: lincopan@usp.br.
Abstract
OBJECTIVES: Klebsiella pneumoniae sequence type 307 (ST307) has emerged as a new high-risk clone worldwide. The aim of this study was to report the draft genome sequence of a multidrug-resistant CTX-M-15-positive K. pneumoniae strain causing urinary tract infection in a companion animal in Brazil. METHODS: Whole-genome sequencing was performed on an Illumina NextSeq platform and the genome was de novo assembled using SPAdes. Data analysis was performed using online tools from the Center for Genomic Epidemiology. RESULTS: The genome size of K. pneumoniae strain PRETA was calculated at 5 450 575 bp, containing a total of 5355 protein-coding sequences. The strain was assigned to the high-risk ST307 and carried the clinically relevant blaCTX-M-15 gene, besides other genes conferring resistance to aminoglycosides, tetracyclines, quinolones, trimethoprim and fosfomycin. CONCLUSION: These data offer novel information for comparative genomic analyses in order to track the transmission dynamics and epidemiology of the newly emerging high-risk clone ST307.
OBJECTIVES:Klebsiella pneumoniae sequence type 307 (ST307) has emerged as a new high-risk clone worldwide. The aim of this study was to report the draft genome sequence of a multidrug-resistant CTX-M-15-positive K. pneumoniae strain causing urinary tract infection in a companion animal in Brazil. METHODS: Whole-genome sequencing was performed on an Illumina NextSeq platform and the genome was de novo assembled using SPAdes. Data analysis was performed using online tools from the Center for Genomic Epidemiology. RESULTS: The genome size of K. pneumoniae strain PRETA was calculated at 5 450 575 bp, containing a total of 5355 protein-coding sequences. The strain was assigned to the high-risk ST307 and carried the clinically relevant blaCTX-M-15 gene, besides other genes conferring resistance to aminoglycosides, tetracyclines, quinolones, trimethoprim and fosfomycin. CONCLUSION: These data offer novel information for comparative genomic analyses in order to track the transmission dynamics and epidemiology of the newly emerging high-risk clone ST307.
Authors: Kira Schmitt; Stefan P Kuster; Katrin Zurfluh; Rahel S Jud; Jane E Sykes; Roger Stephan; Barbara Willi Journal: Antibiotics (Basel) Date: 2021-02-09
Authors: Isabel Carvalho; Nadia Safia Chenouf; José António Carvalho; Ana Paula Castro; Vanessa Silva; Rosa Capita; Carlos Alonso-Calleja; Maria de Lurdes Nunes Enes Dapkevicius; Gilberto Igrejas; Carmen Torres; Patrícia Poeta Journal: PLoS One Date: 2021-05-04 Impact factor: 3.240