Claudine Fournier1, Marta Aires-de-Sousa2, Patrice Nordmann3, Laurent Poirel4. 1. Medical and Molecular Microbiology Unit, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland. 2. Escola Superior de Saúde da Cruz Vermelha Portuguesa, Lisbon, Portugal; Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal. 3. Medical and Molecular Microbiology Unit, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland; INSERM European Unit, University of Fribourg, Fribourg, Switzerland; Swiss National Reference Centre for Emerging Antibiotic Resistance, University of Fribourg, Fribourg, Switzerland; Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland. 4. Medical and Molecular Microbiology Unit, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland; INSERM European Unit, University of Fribourg, Fribourg, Switzerland; Swiss National Reference Centre for Emerging Antibiotic Resistance, University of Fribourg, Fribourg, Switzerland. Electronic address: laurent.poirel@unifr.ch.
Abstract
AIMS: To undertake a prospective analysis of the occurrence of colistin-resistant and extended-spectrum ß-lactamase (ESBL)-producing Enterobacterales colonizing pigs at two farms in Portugal, and to evaluate the putative correlations with usage of different antibiotics. MATERIALS AND METHODS: One hundred and two faecal samples recovered from two different Portuguese pig farms were screened for polymyxin-resistant and ESBL-positive Enterobacterales. The authors had undertaken a study at one of the farms previously, but the use of colistin has since been banned; zinc oxide and amoxicillin are used as prophylactic and curative drugs, respectively, at this farm. The other farm included in this study used zinc oxide alone. RESULTS: Ninety-three ESBL-producing isolates (62 Escherichia coli, 29 Klebsiella pneumoniae, one Enterobacter aerogenes and one Enterobacter cloacae) and 17 colistin-resistant isolates (12 E. coli, four K. pneumoniae and one E. cloacae) were recovered. Among the ESBL producers, the majority (84%) produced CTX-M-15, while the others produced CTX-M-1 or CTX-M-9. Many different strain and plasmid backgrounds were identified, ruling out a massive dissemination of one major clone. In total, 17 colistin-resistant isolates were recovered, all from the first farm. All produced MCR-1, corresponding to 12 E. coli (10 clones) and three K. pneumoniae (two clones). The MCR-1 producers were all recovered from the farm where colistin had been used 2 years previously. CONCLUSION: This study showed a surprisingly high rate of CTX-M-15 producers at two Portuguese pig farms. A link was found between antibiotic selective pressure (ß-lactam or polymyxin) and the corresponding resistance rate.
AIMS: To undertake a prospective analysis of the occurrence of colistin-resistant and extended-spectrum ß-lactamase (ESBL)-producing Enterobacterales colonizing pigs at two farms in Portugal, and to evaluate the putative correlations with usage of different antibiotics. MATERIALS AND METHODS: One hundred and two faecal samples recovered from two different Portuguese pig farms were screened for polymyxin-resistant and ESBL-positive Enterobacterales. The authors had undertaken a study at one of the farms previously, but the use of colistin has since been banned; zinc oxide and amoxicillin are used as prophylactic and curative drugs, respectively, at this farm. The other farm included in this study used zinc oxide alone. RESULTS: Ninety-three ESBL-producing isolates (62 Escherichia coli, 29 Klebsiella pneumoniae, one Enterobacter aerogenes and one Enterobacter cloacae) and 17 colistin-resistant isolates (12 E. coli, four K. pneumoniae and one E. cloacae) were recovered. Among the ESBL producers, the majority (84%) produced CTX-M-15, while the others produced CTX-M-1 or CTX-M-9. Many different strain and plasmid backgrounds were identified, ruling out a massive dissemination of one major clone. In total, 17 colistin-resistant isolates were recovered, all from the first farm. All produced MCR-1, corresponding to 12 E. coli (10 clones) and three K. pneumoniae (two clones). The MCR-1 producers were all recovered from the farm where colistin had been used 2 years previously. CONCLUSION: This study showed a surprisingly high rate of CTX-M-15 producers at two Portuguese pig farms. A link was found between antibiotic selective pressure (ß-lactam or polymyxin) and the corresponding resistance rate.
Authors: Mustafa Sadek; José Manuel Ortiz de la Rosa; Mohamed Abdelfattah Maky; Mohamed Korashe Dandrawy; Patrice Nordmann; Laurent Poirel Journal: Microorganisms Date: 2021-01-19
Authors: Elena González-Fandos; Alba Martínez-Laorden; Ana Abad-Fau; Eloisa Sevilla; Rosa Bolea; María Jesús Serrano; Olga Mitjana; Cristina Bonastre; Alicia Laborda; María Victoria Falceto; Rafael Pagán Journal: Animals (Basel) Date: 2022-03-01 Impact factor: 2.752
Authors: Sarrah Landolsi; Rachid Selmi; Linda Hadjadj; Asma Ben Haj Yahia; Kaouther Ben Romdhane; Lilia Messadi; Jean Marc Rolain Journal: Microbiol Spectr Date: 2022-03-01
Authors: Oscar Mencía-Ares; Raúl Cabrera-Rubio; José Francisco Cobo-Díaz; Avelino Álvarez-Ordóñez; Manuel Gómez-García; Héctor Puente; Paul D Cotter; Fiona Crispie; Ana Carvajal; Pedro Rubio; Héctor Argüello Journal: Microbiome Date: 2020-11-19 Impact factor: 14.650
Authors: Konstantinos Koutsoumanis; Ana Allende; Avelino Álvarez-Ordóñez; Declan Bolton; Sara Bover-Cid; Marianne Chemaly; Robert Davies; Alessandra De Cesare; Lieve Herman; Friederike Hilbert; Roland Lindqvist; Maarten Nauta; Giuseppe Ru; Marion Simmons; Panagiotis Skandamis; Elisabetta Suffredini; Héctor Argüello; Thomas Berendonk; Lina Maria Cavaco; William Gaze; Heike Schmitt; Ed Topp; Beatriz Guerra; Ernesto Liébana; Pietro Stella; Luisa Peixe Journal: EFSA J Date: 2021-06-17