Xiaoying Sun1,2, Shaojuan Yang3,4, Xuechao Feng5, Yaowu Zheng5,6, Jinsong Zhou7,3, Hai Wang7,3, Yucheng Zhang3,8, Hongyan Sun3,9, Chengyan He10,11. 1. Department of Laboratory Medicine, China-Japan Union Hospital of Jilin University, Changchun, 130033, China. sun_xy@jlu.edu.cn. 2. Norman Bethune Health Science Center of Jilin University, Changchun, 130021, China. sun_xy@jlu.edu.cn. 3. Norman Bethune Health Science Center of Jilin University, Changchun, 130021, China. 4. Department of Pathology, China-Japan Union Hospital of Jilin University, Changchun, 130033, China. 5. College of Life Sciences, Northeast Normal University, Changchun, 130024, China. 6. Institute of Cardiovascular Research, University of California, San Francisco, CA, 94101, USA. 7. Department of Laboratory Medicine, China-Japan Union Hospital of Jilin University, Changchun, 130033, China. 8. Department of Science Research Center, China-Japan Union Hospital of Jilin University, Changchun, 130033, China. 9. Department of Tissue Bank, China-Japan Union Hospital of Jilin University, Changchun, 130033, China. 10. Department of Laboratory Medicine, China-Japan Union Hospital of Jilin University, Changchun, 130033, China. cyhe@jlu.edu.cn. 11. Norman Bethune Health Science Center of Jilin University, Changchun, 130021, China. cyhe@jlu.edu.cn.
Abstract
BACKGROUND: To investigate the biological relationship, mechanism between perilipin2 and the occurrence, advancement of gastric carcinoma, and explore the mechanism of lipid metabolism disorder leading to gastric neoplasm, and propose that perilipin2 is presumably considered as a potential molecular biomarker of gastric carcinoma. METHODS: RNA-seq was applied to analyze perilipin2 and differentially expressed genes modulated by perilipin2 in neoplastic tissues of both perilipin2 overexpression and knockdown groups in vivo. The mechanism was discovered and confirmed by Rt-qPCR, immunoblotting, immunohistochemistry, staining and microassay, respectively. Cellular function experiments were performed by flow cytometry, CCK8, clonogenic assay, etc. RESULTS: Overexpression and knockdown of perilipin2 augmented the proliferation and apoptosis of gastric carcinoma cell lines SGC7901 and MGC803, respectively. The neoplastic cells with perilipin2-overexpression obtained more conspicuously rapid growth than knockdown group in vivo, and perilipin2 affected the proliferation and apoptosis of gastric carcinoma cells by modulating the related genes:acyl-coa synthetase long-chain family member 3, arachidonate 15-lipoxygenase, microtubule associated protein 1 light chain 3 alpha, pr/set domain 11 and importin 7 that were participated in Ferroptosis pathway. Moreover, RNA-seq indicated perilipin2 was an indispensable gene and protein in the suppression of Ferroptosis caused by abnormal lipometabolism in gastric carcinoma. CONCLUSION: Our study expounded the facilitation of perilipin2 in regulating the proliferation and apoptosis of gastric carcinoma cells by modification in Ferroptosis pathway, and we interpreted that the mechanism of gastric neoplasm caused by obesity, we also discovered that pr/set domain 11 and importin 7 are novel transcription factors relevant to gastric carcinoma. Furthermore, perilipin2 probably serves not only as a diagnostic biomarker, but also a new therapeutic target.
BACKGROUND: To investigate the biological relationship, mechanism between perilipin2 and the occurrence, advancement of gastric carcinoma, and explore the mechanism of lipid metabolism disorder leading to gastric neoplasm, and propose that perilipin2 is presumably considered as a potential molecular biomarker of gastric carcinoma. METHODS: RNA-seq was applied to analyze perilipin2 and differentially expressed genes modulated by perilipin2 in neoplastic tissues of both perilipin2 overexpression and knockdown groups in vivo. The mechanism was discovered and confirmed by Rt-qPCR, immunoblotting, immunohistochemistry, staining and microassay, respectively. Cellular function experiments were performed by flow cytometry, CCK8, clonogenic assay, etc. RESULTS: Overexpression and knockdown of perilipin2 augmented the proliferation and apoptosis of gastric carcinoma cell lines SGC7901 and MGC803, respectively. The neoplastic cells with perilipin2-overexpression obtained more conspicuously rapid growth than knockdown group in vivo, and perilipin2 affected the proliferation and apoptosis of gastric carcinoma cells by modulating the related genes:acyl-coa synthetase long-chain family member 3, arachidonate 15-lipoxygenase, microtubule associated protein 1 light chain 3 alpha, pr/set domain 11 and importin 7 that were participated in Ferroptosis pathway. Moreover, RNA-seq indicated perilipin2 was an indispensable gene and protein in the suppression of Ferroptosis caused by abnormal lipometabolism in gastric carcinoma. CONCLUSION: Our study expounded the facilitation of perilipin2 in regulating the proliferation and apoptosis of gastric carcinoma cells by modification in Ferroptosis pathway, and we interpreted that the mechanism of gastric neoplasm caused by obesity, we also discovered that pr/set domain 11 and importin 7 are novel transcription factors relevant to gastric carcinoma. Furthermore, perilipin2 probably serves not only as a diagnostic biomarker, but also a new therapeutic target.
Authors: Shashank Masaldan; Ashley I Bush; David Devos; Anne Sophie Rolland; Caroline Moreau Journal: Free Radic Biol Med Date: 2018-09-25 Impact factor: 7.376
Authors: Bo Qiu; Daniel Ackerman; Danielle J Sanchez; Bo Li; Joshua D Ochocki; Alison Grazioli; Ekaterina Bobrovnikova-Marjon; J Alan Diehl; Brian Keith; M Celeste Simon Journal: Cancer Discov Date: 2015-03-31 Impact factor: 39.397
Authors: Sebastian Doll; Bettina Proneth; Yulia Y Tyurina; Elena Panzilius; Sho Kobayashi; Irina Ingold; Martin Irmler; Johannes Beckers; Michaela Aichler; Axel Walch; Holger Prokisch; Dietrich Trümbach; Gaowei Mao; Feng Qu; Hulya Bayir; Joachim Füllekrug; Christina H Scheel; Wolfgang Wurst; Joel A Schick; Valerian E Kagan; José Pedro Friedmann Angeli; Marcus Conrad Journal: Nat Chem Biol Date: 2016-11-14 Impact factor: 15.040