High fluorescence cell count in pleural fluids for malignant effusion screening.

Malignant pleural effusion (MPE) is mainly secondary to pleural metastasis. Its prevalence is 15 to 35% of all the pleural effusions, and the median of survival oscillates between 4 and 6 months, reason why it is very important to know how to diagnose it. The Sysmex XN-350® is an automated hematological analyzer that allows white blood cell count and differentiation, as well as high fluorescence cells (HFC) which includes macrophages, mesothelial and neoplastic cells. For MPE screening, the best combinations obtained were HF-BF# ≥ 17/μL and HF-BF# > 10/μL, both in the absence of heart failure and/or low respiratory infection. The results of this study show that the automated analysis of the pleural fluid with the Sysmex XN-350® analyzer is effective for the screening of the MPE.