Yogaraje Gowda C Varadaiah1,2, Senthilkumar Sivanesan1, Shivananda B Nayak3,4, Kashinath R Thirumalarao4. 1. Department of Research and Development, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, India. 2. Department of Biochemistry, Bangalore Medical College and Research Institute, Bengaluru, India. 3. Department of Preclinical Sciences, Faculty of Medical Sciences, The University of the West Indies, Trinidad, Trinidad and Tobago. 4. Department of Biochemistry, Department of Research and Development, Subbaiah Institute of Medical Sciences, Shivamogga, India.
Abstract
AIM: To find the association between purine metabolites and diabetic complications in rats. MATERIALS AND METHODS: Alloxan was administered to induce diabetes in rats. After 30 days, the levels of uric acid, inosine, xanthine, hypoxanthine and AMP were assessed in both plasma and liver tissues using HPLC technique. RESULTS: A significant increase in xanthine, hypoxanthine, AMP levels (p < .001 and t-value 2.78) and inosine in plasma and liver tissues (p < .05 and t-value 2.11) with a concomitant increase in uric acid levels (p < .001 and t-value 2.80) was observed in diabetic group. CONCLUSION: Purine metabolites like uric acid and other intermediate products of purine metabolism are increased in diabetes. These results can be used in addition or separately in evaluating the progression of diabetes.
AIM: To find the association between purine metabolites and diabetic complications in rats. MATERIALS AND METHODS: Alloxan was administered to induce diabetes in rats. After 30 days, the levels of uric acid, inosine, xanthine, hypoxanthine and AMP were assessed in both plasma and liver tissues using HPLC technique. RESULTS: A significant increase in xanthine, hypoxanthine, AMP levels (p < .001 and t-value 2.78) and inosine in plasma and liver tissues (p < .05 and t-value 2.11) with a concomitant increase in uric acid levels (p < .001 and t-value 2.80) was observed in diabetic group. CONCLUSION: Purine metabolites like uric acid and other intermediate products of purine metabolism are increased in diabetes. These results can be used in addition or separately in evaluating the progression of diabetes.
Authors: Venkatesh L Murthy; Matthew Nayor; Mercedes Carnethon; Jared P Reis; Donald Lloyd-Jones; Norrina B Allen; Robert Kitchen; Paolo Piaggi; Lyn M Steffen; Ramachandran S Vasan; Jane E Freedman; Clary B Clish; Ravi V Shah Journal: Diabetologia Date: 2022-01-18 Impact factor: 10.122