| Literature DB >> 31508976 |
Jan Hegermann1,2, Christoph Wrede1,2, Susanne Fassbender1, Ronja Schliep1, Matthias Ochs3,4, Lars Knudsen1,5, Christian Mühlfeld1,2,5.
Abstract
Generation of three-dimensional (3D) data sets from serial sections of tissues imaged by light microscopy (LM) allows identification of rare structures by morphology or fluorescent labeling. Here, we demonstrate a workflow for correlative LM and electron microscopy (EM) from 3D LM to 3D EM, using the same sectioned material for both methods consecutively. The new approach is easy to reproduce in routine EM laboratories and applicable to a wide range of organs and research questions.Keywords: correlative light and electron microscopy; fluorescence microscopy; focused ion beam scanning electron microscopy; lung; tissue clearing
Mesh：
Year: 2019 PMID： 31508976 DOI： 10.1152/ajplung.00333.2019
Source DB: PubMed Journal: Am J Physiol Lung Cell Mol Physiol ISSN： 1040-0605 Impact factor: 5.464