L Chodari1,2, S Smailnejad1, M Fallahi3, N Khalaji1, V Ghorbanzadeh3,4. 1. Department of Physiology, Urmia University of Medical Sciences, Urmia, Iran. 2. Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran. 3. Drug Applied Research Center of Tabriz, Urmia, Iran. 4. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Abstract
OBJECTIVE: Cardiovascular disorders in diabetes condition arise from increased oxidative stress. Both regular mild exercise and testosterone influence on body's antioxidant system in diabetes. In this study, we evaluated treatment of testosterone and voluntary exercise, alone or together on oxidative stress in the heart and blood of diabetic rats. METHODS: Type 1 diabetes was induced by intraperitoneal injection of 50 mg/kg of streptozotocin in rats. Sixty three rats have been divided into eight groups as follows: Diabetes, diabetes+ testosterone, diabetes+ exercise, diabetes+ testosterone+ exercise, diabetes+ castration, diabetes+ castration+ testosterone, Diabetes+ castration+ exercise, Diabetes+ castration+ exercise+ testosterone. Type 1 diabetes was induced by intraperitoneal injection of 50 mg/kg of streptozotocin in the male Wistar rats and after a week, castration was performed. After 42 days of treatment with testosterone (2 mg/kg/day) or voluntary exercise alone or in combination, SOD, GPX and CAT activities and MDA levels were measured in the blood and heart tissue samples in the groups of study. In the end of study, SOD, GPX and CAT activities and MDA levels were measured in blood and heart tissue samples in the groups of study. RESULTS: SOD, GPX and CAT activities significantly (p<0.05) increased in groups that treated either testosterone or exercise and MDA level significantly (p<0.01) decreased in the blood and heart tissue of diabetic and castrated diabetic rats. Simultaneously, treatment with testosterone and exercise had a synergistic effect on antioxidant enzymes level in diabetic and diabetic castrated rats. In the castrated animals with diabetes, SOD, GPX and CAT activities significantly decreased (p<0.05) and MDA levels significantly increased (p<0.05) in blood and heart tissue. CONCLUSION: Voluntary exercise and testosterone alone or together heightened body's antioxidant system and were able to reduce the MDA levels in blood and heart of diabetic and castrated diabetic rats.
OBJECTIVE: Cardiovascular disorders in diabetes condition arise from increased oxidative stress. Both regular mild exercise and testosterone influence on body's antioxidant system in diabetes. In this study, we evaluated treatment of testosterone and voluntary exercise, alone or together on oxidative stress in the heart and blood of diabetic rats. METHODS: Type 1 diabetes was induced by intraperitoneal injection of 50 mg/kg of streptozotocin in rats. Sixty three rats have been divided into eight groups as follows: Diabetes, diabetes+ testosterone, diabetes+ exercise, diabetes+ testosterone+ exercise, diabetes+ castration, diabetes+ castration+ testosterone, Diabetes+ castration+ exercise, Diabetes+ castration+ exercise+ testosterone. Type 1 diabetes was induced by intraperitoneal injection of 50 mg/kg of streptozotocin in the male Wistar rats and after a week, castration was performed. After 42 days of treatment with testosterone (2 mg/kg/day) or voluntary exercise alone or in combination, SOD, GPX and CAT activities and MDA levels were measured in the blood and heart tissue samples in the groups of study. In the end of study, SOD, GPX and CAT activities and MDA levels were measured in blood and heart tissue samples in the groups of study. RESULTS: SOD, GPX and CAT activities significantly (p<0.05) increased in groups that treated either testosterone or exercise and MDA level significantly (p<0.01) decreased in the blood and heart tissue of diabetic and castrated diabetic rats. Simultaneously, treatment with testosterone and exercise had a synergistic effect on antioxidant enzymes level in diabetic and diabetic castrated rats. In the castrated animals with diabetes, SOD, GPX and CAT activities significantly decreased (p<0.05) and MDA levels significantly increased (p<0.05) in blood and heart tissue. CONCLUSION: Voluntary exercise and testosterone alone or together heightened body's antioxidant system and were able to reduce the MDA levels in blood and heart of diabetic and castrated diabetic rats.
Authors: Susana Sangiao-Alvarellos; Sergio Polakof; Francisco J Arjona; Angel García-López; María P Martín del Río; Gonzalo Martínez-Rodríguez; Jesús M Míguez; Juan M Mancera; José L Soengas Journal: Gen Comp Endocrinol Date: 2006-06-12 Impact factor: 2.822
Authors: Isaac Túnez; Montserrat Feijóo; Juan A Collado; Francisco J Medina; José Peña; María del C Muñoz; Ignacio Jimena; Francisco Franco; Ignacio Rueda; Jordi Muntané; Pedro Montilla Journal: Life Sci Date: 2007-01-09 Impact factor: 5.037
Authors: Eveline W C M van Dam; Jacqueline M Dekker; Eef G W M Lentjes; Fred P T H M Romijn; Yvo M Smulders; Wendy J Post; Johannes A Romijn; H Michiel J Krans Journal: Diabetes Care Date: 2003-06 Impact factor: 19.112
Authors: D G de Lima; C F da Silva; A G Freitas; O L P da Silva; F M A de Souza; M J S Bortolini; N Penha-Silva; F G A Santos; Y K de Carvalho; V E Valenti; R P M Silva Journal: Acta Endocrinol (Buchar) Date: 2021 Jul-Sep Impact factor: 0.877