María Pérez-Vázquez1,2, Pedro J Sola Campoy1, Adriana Ortega1,2, Verónica Bautista1,2, Sara Monzón3, Guillermo Ruiz-Carrascoso2,4, Jesus Mingorance2,4, Eva M González-Barberá5, Concepción Gimeno6, Belén Aracil1,2, David Sáez1,2, Noelia Lara1,2, Sara Fernández1,2, Juan José González-López2,7, José Campos1,2, Robert A Kingsley8,9, Gordon Dougan8, Jesús Oteo-Iglesias1,2. 1. Laboratorio de Referencia e Investigación en Resistencia a Antibióticos e Infecciones Relacionadas con la Asistencia Sanitaria, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain. 2. Spanish Network for Research in Infectious Diseases (REIPI RD12/0015 and REIPI RD16/0016), Instituto de Salud Carlos III, Madrid, Spain. 3. Unidad de Bioinformátia (BU-ISCIII), Instituto de Salud Carlos III, Majadahonda, Madrid, Spain. 4. Servicio de Microbiología, Hospital Universitario La Paz-idiPAZ, Madrid, Spain. 5. Servicio de Microbiología, Hospital Universitario La Fe, Valencia, Spain. 6. Servicio de Microbiología, Hospital General Universitario de Valencia, Valencia, Spain. 7. Clinical Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain. 8. The Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK. 9. Quadram Institute Bioscience, Colney, Norwich, UK.
Abstract
OBJECTIVES: NDM carbapenemases have spread worldwide. However, little information exists about the impact of NDM-producing Enterobacteriaceae in Spain. By WGS, we sought to elucidate the population structure of NDM-like-producing Klebsiella pneumoniae and Escherichia coli in Spain and to determine the plasmids harbouring blaNDM-like genes. METHODS: High-resolution SNP typing, core-genome MLST and plasmid reconstruction (PlasmidID) were performed on 59 NDM-like-producing K. pneumoniae and 8 NDM-like-producing E. coli isolated over an 8 year period in Spain. RESULTS: Five major epidemic clones of NDM-producing K. pneumoniae caused five important nationwide outbreaks: ST437/NDM-7, ST437/NDM-1, ST147/NDM-1, ST11/NDM-1 and ST101/NDM-1; in contrast, the spread of NDM-producing E. coli was polyclonal. Three blaNDM types were identified: blaNDM-1, 61.2%; blaNDM-7, 32.8%; and blaNDM-5, 6%. Five K. pneumoniae isolates co-produced other carbapenemases (three blaOXA-48 and two blaVIM-1). The average number of acquired resistance genes was higher in K. pneumoniae than in E. coli. The plasmids encoding blaNDM-like genes belonged to IncFII, IncFIB, IncX3, IncR, IncN and IncC types, of which IncF, IncR and IncC were associated with MDR. The genetic surroundings of blaNDM-like genes showed a highly variable region upstream of ISAba125. CONCLUSIONS: In recent years NDM-producing K. pneumoniae and E. coli have emerged in Spain; the spread of a few high-risk K. pneumoniae clones such as ST437/NDM-7, ST437/NDM-1, ST147/NDM-1, ST11/NDM-1 and ST101/NDM-1 have caused several interregional outbreaks. In contrast, the spread of NDM-producing E. coli has been polyclonal. Plasmid types IncFII, IncFIB, IncX3, IncR, IncN and IncC carried blaNDM, and the same IncX3 plasmid was detected in K. pneumoniae and E. coli.
OBJECTIVES: NDM carbapenemases have spread worldwide. However, little information exists about the impact of NDM-producing Enterobacteriaceae in Spain. By WGS, we sought to elucidate the population structure of NDM-like-producing Klebsiella pneumoniae and Escherichia coli in Spain and to determine the plasmids harbouring blaNDM-like genes. METHODS: High-resolution SNP typing, core-genome MLST and plasmid reconstruction (PlasmidID) were performed on 59 NDM-like-producing K. pneumoniae and 8 NDM-like-producing E. coli isolated over an 8 year period in Spain. RESULTS: Five major epidemic clones of NDM-producing K. pneumoniae caused five important nationwide outbreaks: ST437/NDM-7, ST437/NDM-1, ST147/NDM-1, ST11/NDM-1 and ST101/NDM-1; in contrast, the spread of NDM-producing E. coli was polyclonal. Three blaNDM types were identified: blaNDM-1, 61.2%; blaNDM-7, 32.8%; and blaNDM-5, 6%. Five K. pneumoniae isolates co-produced other carbapenemases (three blaOXA-48 and two blaVIM-1). The average number of acquired resistance genes was higher in K. pneumoniae than in E. coli. The plasmids encoding blaNDM-like genes belonged to IncFII, IncFIB, IncX3, IncR, IncN and IncC types, of which IncF, IncR and IncC were associated with MDR. The genetic surroundings of blaNDM-like genes showed a highly variable region upstream of ISAba125. CONCLUSIONS: In recent years NDM-producing K. pneumoniae and E. coli have emerged in Spain; the spread of a few high-risk K. pneumoniae clones such as ST437/NDM-7, ST437/NDM-1, ST147/NDM-1, ST11/NDM-1 and ST101/NDM-1 have caused several interregional outbreaks. In contrast, the spread of NDM-producing E. coli has been polyclonal. Plasmid types IncFII, IncFIB, IncX3, IncR, IncN and IncC carried blaNDM, and the same IncX3 plasmid was detected in K. pneumoniae and E. coli.
Authors: Weverton de Oliveira Alves; Alexsandra Maria Lima Scavuzzi; Elizabeth Maria Bispo Beltrão; Érica Maria de Oliveira; Crhisllane Rafaele Dos Santos Vasconcelos; Antônio Mauro Rezende; Ana Catarina de Souza Lopes Journal: Arch Microbiol Date: 2022-07-05 Impact factor: 2.552
Authors: Javier E Cañada-García; Zaira Moure; Pedro J Sola-Campoy; Mercedes Delgado-Valverde; María E Cano; Desirèe Gijón; Mónica González; Irene Gracia-Ahufinger; Nieves Larrosa; Xavier Mulet; Cristina Pitart; Alba Rivera; Germán Bou; Jorge Calvo; Rafael Cantón; Juan José González-López; Luis Martínez-Martínez; Ferran Navarro; Antonio Oliver; Zaira R Palacios-Baena; Álvaro Pascual; Guillermo Ruiz-Carrascoso; Jordi Vila; Belén Aracil; María Pérez-Vázquez; Jesús Oteo-Iglesias Journal: Front Microbiol Date: 2022-06-30 Impact factor: 6.064
Authors: Sophia David; Victoria Cohen; Sandra Reuter; Anna E Sheppard; Tommaso Giani; Julian Parkhill; Gian Maria Rossolini; Edward J Feil; Hajo Grundmann; David M Aanensen Journal: Proc Natl Acad Sci U S A Date: 2020-09-23 Impact factor: 11.205