Jinming Cen1, Qingyuan Xiong2, Xili Yang3, Jun Guo3, Xing-Dong Xiong4, Aidong Zhang5. 1. Department of Cardiovascular Medicine, The First Affiliated Hospital of Jinan University, China; Department of Cardiovascular Medicine, Foshan Municipal First People's Hospital, China. 2. Department of Clinical laboratory, The Chancheng District Central Hospital of Foshan, China. 3. Department of Cardiovascular Medicine, The First Affiliated Hospital of Jinan University, China. 4. Institute of Aging Research, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, China. 5. Department of Cardiovascular Medicine, The First Affiliated Hospital of Jinan University, China. Electronic address: 289124834@qq.com.
Abstract
OBJECTIVES: Accumulating evidences have shown that polymorphisms in miRNA genes play an important role in the susceptibility to coronary artery disease (CAD). A potentially functional polymorphism rs4938723, which located within the promoter region of pri-miR-34b/c, may affect the expression of miR-34b/c. To date, the role of genetic variant in pri-miR-34b/c on CAD risk is still unknown. Here we aimed to evaluate the association of Pri-miR-34b/c rs4938723 polymorphism with individual susceptibility to CAD in a Chinese Han population. METHODS: Genotyping was performed in a case-control study of 563 patients and 646 controls using polymerase chain reaction-ligase detection reaction (PCR-LDR) method. The association of rs4938723 with CAD risk was evaluated using logistic regression analysis with SPSS software. RESULTS: We found that the C allele of pri-miR-34b/c rs4938723 was significantly associated with a decreased risk of CAD when compared with the T allele (OR = 0.76, 95% CI = 0.62-0.95, p = 0.015). Consistently, compared with those carrying TT genotype, the CC homozygotes displayed significantly reduced risk for CAD (OR = 0.54, 95% CI = 0.32-0.91, p = 0.021). Similar trend of the reduced risk for CAD was detected when the CT and CC genotypes were combined (OR = 0.75, 95% CI = 0.57-0.99, p = 0.044). Stratified analysis of pri-miR-34b/c rs4938723 revealed a more significant association of C allele with decreased CAD risk among older subjects, male and non-smokers. CONCLUSIONS: Our findings suggest that the pri-miR-34b/c rs4938723 polymorphism is associated with CAD susceptibility in the Chinese Han population. Further studies are warranted to confirm the general validity of our findings.
OBJECTIVES: Accumulating evidences have shown that polymorphisms in miRNA genes play an important role in the susceptibility to coronary artery disease (CAD). A potentially functional polymorphism rs4938723, which located within the promoter region of pri-miR-34b/c, may affect the expression of miR-34b/c. To date, the role of genetic variant in pri-miR-34b/c on CAD risk is still unknown. Here we aimed to evaluate the association of Pri-miR-34b/c rs4938723 polymorphism with individual susceptibility to CAD in a Chinese Han population. METHODS: Genotyping was performed in a case-control study of 563 patients and 646 controls using polymerase chain reaction-ligase detection reaction (PCR-LDR) method. The association of rs4938723 with CAD risk was evaluated using logistic regression analysis with SPSS software. RESULTS: We found that the C allele of pri-miR-34b/c rs4938723 was significantly associated with a decreased risk of CAD when compared with the T allele (OR = 0.76, 95% CI = 0.62-0.95, p = 0.015). Consistently, compared with those carrying TT genotype, the CC homozygotes displayed significantly reduced risk for CAD (OR = 0.54, 95% CI = 0.32-0.91, p = 0.021). Similar trend of the reduced risk for CAD was detected when the CT and CC genotypes were combined (OR = 0.75, 95% CI = 0.57-0.99, p = 0.044). Stratified analysis of pri-miR-34b/c rs4938723 revealed a more significant association of C allele with decreased CAD risk among older subjects, male and non-smokers. CONCLUSIONS: Our findings suggest that the pri-miR-34b/c rs4938723 polymorphism is associated with CAD susceptibility in the Chinese Han population. Further studies are warranted to confirm the general validity of our findings.