Caroline Truntzer1, Nicolas Isambert2, Laurent Arnould3, Sylvain Ladoire4, Francois Ghiringhelli5. 1. Platform of Transfer in Biology of Cancer, Georges Francois Leclerc Cancer Center, 1 Rue Du Professeur Marion, 21000 Dijon, France; GIMI Genetic and Immunology Medical Institute, 21000 Dijon, France. Electronic address: ctruntzer@cgfl.fr. 2. Department of Medical Oncology, Georges Francois Leclerc Cancer Center, 1 Rue Du Professeur Marion, 21000 Dijon, France. Electronic address: nisambert@cgf.fr. 3. Department of Medical Oncology, Georges Francois Leclerc Cancer Center, 1 Rue Du Professeur Marion, 21000 Dijon, France; Department of Pathology, Georges Francois Leclerc Cancer Center, 1 Rue Du Professeur Marion, 21000 Dijon, France. Electronic address: larnould@cgfl.fr. 4. Platform of Transfer in Biology of Cancer, Georges Francois Leclerc Cancer Center, 1 Rue Du Professeur Marion, 21000 Dijon, France; GIMI Genetic and Immunology Medical Institute, 21000 Dijon, France; Department of Medical Oncology, Georges Francois Leclerc Cancer Center, 1 Rue Du Professeur Marion, 21000 Dijon, France; INSERM U1231, Dijon, France; Univ. Bourgogne Franche-Comté, Dijon, France. Electronic address: sladoire@cgfl.fr. 5. Platform of Transfer in Biology of Cancer, Georges Francois Leclerc Cancer Center, 1 Rue Du Professeur Marion, 21000 Dijon, France; GIMI Genetic and Immunology Medical Institute, 21000 Dijon, France; Department of Medical Oncology, Georges Francois Leclerc Cancer Center, 1 Rue Du Professeur Marion, 21000 Dijon, France; INSERM U1231, Dijon, France; Univ. Bourgogne Franche-Comté, Dijon, France. Electronic address: fghiringhelli@cgfl.fr.
Abstract
PURPOSE: Tumour-infiltrating lymphocyte (TIL) detection by histology is associated with outcomes in breast cancer; nevertheless, analysis standardisation is difficult. We determined whether transcriptomic data could generate a genomic signature that estimated TIL infiltrates and determined patient prognosis in localised breast cancer. EXPERIMENTAL DESIGN: Using 1928 transcriptomic profiles of pure cells, we generated a genetic signature specific to lymphocyte, myeloid, stromal and cancer cells. We then computed a score based on this signature and tested the association between the score and the TILs estimated for patients in an adjuvant setting from public and private data sets. We tested the capacity of the transcriptomic RNA TIL score to predict disease-free survival (DFS) or overall survival (OS) through multivariate Cox models adjusted for classical clinical variables and PAM50 molecular classification in two public data sets (Carte d'Identité des Tumeurs [CIT], n = 530; Metabric, n = 1832). RESULTS: A high RNA TIL score was significantly associated with the presence of a high level of TILs as assessed by histology. The score was also associated with DFS and OS in multivariate Cox models adjusted for molecular and clinical variables (CIT: OS hazard ratio [HR] = 0.15 [0.04, 0.61], p-value = 0.007; DFS: 0.27 [0.08, 0.8] p-value = 0.02; Metabric: OS HR = 0.87 [0.77, 0.97], p-value = 0.01). The association between the RNA TIL score and survival was tested by univariate analysis in each molecular subgroup; the RNA TIL score was associated with survival only in basal-like tumours. CONCLUSIONS: Determination of the TIL rate using a transcriptomic signature is feasible and has a high prognostic value in patients with basal-like tumours in an adjuvant setting.
PURPOSE:Tumour-infiltrating lymphocyte (TIL) detection by histology is associated with outcomes in breast cancer; nevertheless, analysis standardisation is difficult. We determined whether transcriptomic data could generate a genomic signature that estimated TIL infiltrates and determined patient prognosis in localised breast cancer. EXPERIMENTAL DESIGN: Using 1928 transcriptomic profiles of pure cells, we generated a genetic signature specific to lymphocyte, myeloid, stromal and cancer cells. We then computed a score based on this signature and tested the association between the score and the TILs estimated for patients in an adjuvant setting from public and private data sets. We tested the capacity of the transcriptomic RNA TIL score to predict disease-free survival (DFS) or overall survival (OS) through multivariate Cox models adjusted for classical clinical variables and PAM50 molecular classification in two public data sets (Carte d'Identité des Tumeurs [CIT], n = 530; Metabric, n = 1832). RESULTS: A high RNA TIL score was significantly associated with the presence of a high level of TILs as assessed by histology. The score was also associated with DFS and OS in multivariate Cox models adjusted for molecular and clinical variables (CIT: OS hazard ratio [HR] = 0.15 [0.04, 0.61], p-value = 0.007; DFS: 0.27 [0.08, 0.8] p-value = 0.02; Metabric: OS HR = 0.87 [0.77, 0.97], p-value = 0.01). The association between the RNA TIL score and survival was tested by univariate analysis in each molecular subgroup; the RNA TIL score was associated with survival only in basal-like tumours. CONCLUSIONS: Determination of the TIL rate using a transcriptomic signature is feasible and has a high prognostic value in patients with basal-like tumours in an adjuvant setting.
Authors: Jian Lei; Zhen-Yu He; Jun Wang; Min Hu; Ping Zhou; Chen-Lu Lian; Li Hua; San-Gang Wu; Juan Zhou Journal: Cancer Med Date: 2021-03-19 Impact factor: 4.452
Authors: Laurys Boudin; A de Nonneville; Pascal Finetti; Léna Mescam; A Le Cesne; Antoine Italiano; Jean-Yves Blay; Daniel Birnbaum; Emilie Mamessier; François Bertucci Journal: J Transl Med Date: 2022-10-11 Impact factor: 8.440