Literature DB >> 3149398

The cloning of the rorB gene of Escherichia coli.

P G Debenham1, M B Webb, J Law.   

Abstract

A segment of the Escherichia coli genome which complements the ionising radiation sensitivity of the rorB mutation was cloned into pBR322. This DNA segment also complements the mitomycin C sensitivity of the rorB mutation. The gene was subcloned until defined in a fragment of 1.05 kb. Only one gene product, a protein of approximately 16.5 kDa, was found on maxicell analysis of the various subclones. Iso-electric focusing of this gene product suggests it may function in a complex.

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Year:  1988        PMID： 3149398     DOI： 10.1007/bf00331318

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  16 in total

1.  The recN locus of Escherichia coli K12: molecular analysis and identification of the gene product.

Authors:  S M Picksley; S J Morton; R G Lloyd
Journal:  Mol Gen Genet       Date:  1985

2.  rorA mutation of Escherichia coli K-12 affects the recB subunit of exonuclease V.

Authors:  B W Glickman
Journal:  J Bacteriol       Date:  1979-01       Impact factor: 3.490

3.  Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.

Authors:  D B Clewell; D R Helinski
Journal:  Biochemistry       Date:  1970-10-27       Impact factor: 3.162

4.  A complementation analysis of the restriction and modification of DNA in Escherichia coli.

Authors:  H W Boyer; D Roulland-Dussoix
Journal:  J Mol Biol       Date:  1969-05-14       Impact factor: 5.469

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.

Authors:  W B Wood
Journal:  J Mol Biol       Date:  1966-03       Impact factor: 5.469

7.  Repair of DNA double-strand breaks in Escherichia coli K12 requires a functional recN product.

Authors:  S M Picksley; P V Attfield; R G Lloyd
Journal:  Mol Gen Genet       Date:  1984

8.  Identification of a protein of 15,000 daltons related to isoleucine-valine biosynthesis in Escherichia coli K-12.

Authors:  J E Gray; J W Wallen; D H Calhoun
Journal:  J Bacteriol       Date:  1982-07       Impact factor: 3.490

9.  Physical and genetic localization of ilv regulatory sites in lambda ilv bacteriophages.

Authors:  J E Gray; D C Bennett; H E Umbarger; D H Calhoun
Journal:  J Bacteriol       Date:  1982-03       Impact factor: 3.490

10.  The use of a cloned bacterial gene to study mutation in mammalian cells.

Authors:  J Thacker; P G Debenham; A Stretch; M B Webb
Journal:  Mutat Res       Date:  1983-09       Impact factor: 2.433
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  2 in total

Review 1.  Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Authors:  M K Berlyn
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

2.  The isolation and preliminary characterisation of a novel Escherichia coli mutant rorB with enhanced sensitivity to ionising radiation.

Authors:  P G Debenham; M B Webb
Journal:  Mol Gen Genet       Date:  1988-12
  2 in total

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