Diqiu Liu1,2, Qin Zhao3,4, Xiuming Cui3,4, Rui Chen3,4, Xin Li3,4, Bingling Qiu3,4, Feng Ge3,4. 1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Number 727 Jing Ming South Road, Chenggong District, Kunming, 650500, Yunnan, China. Diqiuliu@126.com. 2. Yunnan Provincial Key Laboratory of Panax Notoginseng, Kunming, 650500, Yunnan, China. Diqiuliu@126.com. 3. Faculty of Life Science and Technology, Kunming University of Science and Technology, Number 727 Jing Ming South Road, Chenggong District, Kunming, 650500, Yunnan, China. 4. Yunnan Provincial Key Laboratory of Panax Notoginseng, Kunming, 650500, Yunnan, China.
Abstract
BACKGROUND: Panax notoginseng is a famous Chinese herbal medicine, but the root rot disease mainly caused by Fusarium solani severely reduces the yield and quality of its medicinal materials. OBJECTIVE: The defense priming in P. notoginseng through exogenous application of signaling molecule will supply theoretical support for the exogenous regulation of disease resistance in P. notoginseng. METHODS: In this study, the exogenous application of methyl jasmonate (MeJA) increased P. notoginseng's resistance to F. solani. Furthermore, the P. notoginseng transcriptome during F. solani infection was investigated through next-generation sequencing to uncover the resistance mechanism of P. notogingseng induced by MeJA. RESULTS: The de novo assembly of transcriptome sequences produced 80,551 unigenes, and 36,771 of these unigenes were annotated by at least one database. A differentially expressed gene analysis revealed that a large number of genes related to terpenoid backbone biosynthesis, phenylalanine metabolism, and plant-pathogen interactions were predominantly up-regulated by MeJA. Moreover, jasmonic acid (JA) biosynthesis-related genes and the JA signaling pathway genes, such as linoleate 13S-lipoxygenase, allene oxide cyclase, allene oxide synthase, TIFY, defensin, and pathogenesis-related proteins, showed increased transcriptional levels after inoculation with F. solani. Notably, according to the gene expression analysis, JA and ethylene signaling pathways may act synergistically to positively regulate the defense responses of P. notoginseng to F. solani. CONCLUSION: JA signaling appears to play a vital role in P. notoginseng responses to F. solani infection, which will be helpful in improving the disease resistance of P. notoginseng cultivars as well as in developing an environmentally friendly biological control method for root rot disease.
BACKGROUND:Panax notoginseng is a famous Chinese herbal medicine, but the root rot disease mainly caused by Fusarium solani severely reduces the yield and quality of its medicinal materials. OBJECTIVE: The defense priming in P. notoginseng through exogenous application of signaling molecule will supply theoretical support for the exogenous regulation of disease resistance in P. notoginseng. METHODS: In this study, the exogenous application of methyl jasmonate (MeJA) increased P. notoginseng's resistance to F. solani. Furthermore, the P. notoginseng transcriptome during F. solaniinfection was investigated through next-generation sequencing to uncover the resistance mechanism of P. notogingseng induced by MeJA. RESULTS: The de novo assembly of transcriptome sequences produced 80,551 unigenes, and 36,771 of these unigenes were annotated by at least one database. A differentially expressed gene analysis revealed that a large number of genes related to terpenoid backbone biosynthesis, phenylalanine metabolism, and plant-pathogen interactions were predominantly up-regulated by MeJA. Moreover, jasmonic acid (JA) biosynthesis-related genes and the JA signaling pathway genes, such as linoleate 13S-lipoxygenase, allene oxide cyclase, allene oxide synthase, TIFY, defensin, and pathogenesis-related proteins, showed increased transcriptional levels after inoculation with F. solani. Notably, according to the gene expression analysis, JA and ethylene signaling pathways may act synergistically to positively regulate the defense responses of P. notoginseng to F. solani. CONCLUSION:JA signaling appears to play a vital role in P. notoginseng responses to F. solaniinfection, which will be helpful in improving the disease resistance of P. notoginseng cultivars as well as in developing an environmentally friendly biological control method for root rot disease.
Authors: Francisco Amil-Ruiz; José Garrido-Gala; José Gadea; Rosario Blanco-Portales; Antonio Muñoz-Mérida; Oswaldo Trelles; Berta de Los Santos; Francisco T Arroyo; Ana Aguado-Puig; Fernando Romero; José-Ángel Mercado; Fernando Pliego-Alfaro; Juan Muñoz-Blanco; José L Caballero Journal: Front Plant Sci Date: 2016-07-15 Impact factor: 5.753
Authors: Manfred G Grabherr; Brian J Haas; Moran Yassour; Joshua Z Levin; Dawn A Thompson; Ido Amit; Xian Adiconis; Lin Fan; Raktima Raychowdhury; Qiandong Zeng; Zehua Chen; Evan Mauceli; Nir Hacohen; Andreas Gnirke; Nicholas Rhind; Federica di Palma; Bruce W Birren; Chad Nusbaum; Kerstin Lindblad-Toh; Nir Friedman; Aviv Regev Journal: Nat Biotechnol Date: 2011-05-15 Impact factor: 54.908