Manuel Toledano1, José L Gutierrez-Pérez2, Aida Gutierrez-Corrales2, María A Serrera-Figallo2, Manuel Toledano-Osorio1, Juan I Rosales-Leal1, Mariano Aguilar3, Raquel Osorio4, Daniel Torres-Lagares2. 1. Dental School, Colegio Máximo, Research Institute IBS, University of Granada, Campus de Cartuja s/n, 18017, Granada, Spain. 2. Faculty of Dentistry, Oral Surgery Section, University of Sevilla, Avicena s/n, 41009, Sevilla, Spain. 3. Faculty of Medicine, Biopathology and Medicine Regenerative Institute (IBIMER, CIBM), University of Granada, Avda. de la Investigación 11, 18016, Granada, Spain. 4. Dental School, Colegio Máximo, Research Institute IBS, University of Granada, Campus de Cartuja s/n, 18017, Granada, Spain. rosorio@ugr.es.
Abstract
OBJECTIVE: The aim of this study was to evaluate the bone-regeneration efficiency of novel polymeric nanostructured membranes and the effect of zinc, calcium, titanium, and bone morpho-protein loading on membranes, through an in vivo rabbit model. MATERIAL AND METHODS: Nanostructured membranes of methylmethacrylate were loaded with zinc, calcium, TiO2 nanoparticles, and bone-morphogenetic protein (BMP). These membranes covered the bone defects prepared on the skulls of six rabbits. Animals were sacrificed 6 weeks after surgery. Micro computed tomography was used to evaluate bone architecture through BoneJ pluging and ImageJ script. Three histological processing of samples, including von Kossa silver nitrate, toluidine blue, and fluorescence by the deposition of calcein were utilized. RESULTS: Zn-membranes (Zn-Ms) promoted the highest amount of new bone and higher bone perimeter than both unloaded and Ti-membranes (Ti-Ms). Ca-membranes (Ca-Ms) attained higher osteoid perimeter and bone perimeter than Zn-Ms. The skeleton analysis showed that Zn-Ms produced more branches and junctions at the trabecular bone than BMP-loaded membranes (BMP-Ms). Samples treated with Ti-Ms showed less bone formation and bony bridging processes. Both Zn-Ms and Ca-Ms achieved higher number of osteoblasts than the control group. BMP-Ms and Ca-Ms originated higher number of blood vessels than Ti-Ms and control group. CONCLUSIONS: Zn incorporation in novel nanostructured membranes provided the highest regenerative efficiency for bone healing at the rabbit calvarial defects. CLINICAL RELEVANCE: Zn-Ms promoted osteogenesis and enhanced biological activity, as mineralized and osteoid new bone with multiple interconnected ossified trabeculae appeared in close contact with the membrane.
OBJECTIVE: The aim of this study was to evaluate the bone-regeneration efficiency of novel polymeric nanostructured membranes and the effect of zinc, calcium, titanium, and bone morpho-protein loading on membranes, through an in vivo rabbit model. MATERIAL AND METHODS: Nanostructured membranes of methylmethacrylate were loaded with zinc, calcium, TiO2 nanoparticles, and bone-morphogenetic protein (BMP). These membranes covered the bone defects prepared on the skulls of six rabbits. Animals were sacrificed 6 weeks after surgery. Micro computed tomography was used to evaluate bone architecture through BoneJ pluging and ImageJ script. Three histological processing of samples, including von Kossasilver nitrate, toluidine blue, and fluorescence by the deposition of calcein were utilized. RESULTS:Zn-membranes (Zn-Ms) promoted the highest amount of new bone and higher bone perimeter than both unloaded and Ti-membranes (Ti-Ms). Ca-membranes (Ca-Ms) attained higher osteoid perimeter and bone perimeter than Zn-Ms. The skeleton analysis showed that Zn-Ms produced more branches and junctions at the trabecular bone than BMP-loaded membranes (BMP-Ms). Samples treated with Ti-Ms showed less bone formation and bony bridging processes. Both Zn-Ms and Ca-Ms achieved higher number of osteoblasts than the control group. BMP-Ms and Ca-Ms originated higher number of blood vessels than Ti-Ms and control group. CONCLUSIONS:Zn incorporation in novel nanostructured membranes provided the highest regenerative efficiency for bone healing at the rabbit calvarial defects. CLINICAL RELEVANCE: Zn-Ms promoted osteogenesis and enhanced biological activity, as mineralized and osteoid new bone with multiple interconnected ossified trabeculae appeared in close contact with the membrane.
Entities:
Keywords:
Bone regeneration; Non-resorbable polymer; Scaffold; Zinc
Authors: Kacper Kroczek; Paweł Turek; Damian Mazur; Jacek Szczygielski; Damian Filip; Robert Brodowski; Krzysztof Balawender; Łukasz Przeszłowski; Bogumił Lewandowski; Stanisław Orkisz; Artur Mazur; Grzegorz Budzik; Józef Cebulski; Mariusz Oleksy Journal: Polymers (Basel) Date: 2022-04-09 Impact factor: 4.967
Authors: Manuel Toledano; Marta Vallecillo-Rivas; María T Osorio; Esther Muñoz-Soto; Manuel Toledano-Osorio; Cristina Vallecillo; Raquel Toledano; Christopher D Lynch; María-Angeles Serrera-Figallo; Raquel Osorio Journal: Polymers (Basel) Date: 2021-05-29 Impact factor: 4.329