Jun Xiong 1 , Huijun Wang 2 , Feng Mu 1 , Zhanxue Liu 1 , Yin Bao 2 , Yongxing Sun 1 Show Affiliations »
Abstract
PURPOSE: Research has shown that exposure to anesthesia might increase the risks of cognitive impairments and learning difficulties. MiR-125b-5p contributed to anesthesia-induced hippocampal apoptosis. However, the role of miR-125b-5p in sevoflurane-induced cognitive impairments remains unclear. METHODS: Firstly, sevoflurane was used to establish a rat model and cognitive impairment was detected by the Morris water maze (MWM) test. The hippocampus was observed by HE staining. The lentivirus-miR-125b-5p antagomiR was transfected into rats to decrease miR-125b-5p. The interaction between miR-125b-5p and LIM domain kinase 1 (LIMK1) was confirmed by the luciferase reporter assay. The mRNA and expression levels of related genes and mRNA were examined by the Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) and western blot. RESULTS: Sevoflurane induced the cognitive dysfunction presenting with longer latency time and few platform crossings in rats. Moreover, miR-125b-5p was observed to be up-regulated in both sevoflurane-anesthesia rats and sevoflurane-treated SH-SY5Y cells. More importantly, a decrease in miR-125b-5p could prevent sevoflurane-induced hippocampal apoptosis and inflammation in rats. Moreover, LIMK1 was the target gene of miR-125b-5p. Interestingly, si-LIMK1 could restore the sevoflurane-induced cell apoptosis in SH-SY5Y cells, which was alleviated by miR-125b-5p inhibitor. Finally, the miR-125b-5p inhibitor shortened the time to find the platform and increased the number of platform crossings compared to sevoflurane-anesthesia rats in the Morris water maze test. At the same time, the expression of LIMK1 was dramatically increased. CONCLUSION: Altogether, these findings suggested that miR-125b-5p inhibitor could protect against the sevoflurane-induced cognitive impairments by targeting LIMK1. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.
PURPOSE: Research has shown that exposure to anesthesia might increase the risks of cognitive impairments and learning difficulties. MiR-125b-5p contributed to anesthesia-induced hippocampal apoptosis. However, the role of miR-125b-5p in sevoflurane -induced cognitive impairments remains unclear. METHODS: Firstly, sevoflurane was used to establish a rat model and cognitive impairment was detected by the Morris water maze (MWM) test. The hippocampus was observed by HE staining. The lentivirus-miR-125b-5p antagomiR was transfected into rats to decrease miR-125b-5p . The interaction between miR-125b-5p and LIM domain kinase 1 (LIMK1 ) was confirmed by the luciferase reporter assay. The mRNA and expression levels of related genes and mRNA were examined by the Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) and western blot. RESULTS: Sevoflurane induced the cognitive dysfunction presenting with longer latency time and few platform crossings in rats . Moreover, miR-125b-5p was observed to be up-regulated in both sevoflurane -anesthesia rats and sevoflurane -treated SH-SY5Y cells. More importantly, a decrease in miR-125b-5p could prevent sevoflurane -induced hippocampal apoptosis and inflammation in rats . Moreover, LIMK1 was the target gene of miR-125b-5p . Interestingly, si-LIMK1 could restore the sevoflurane -induced cell apoptosis in SH-SY5Y cells, which was alleviated by miR-125b-5p inhibitor. Finally, the miR-125b-5p inhibitor shortened the time to find the platform and increased the number of platform crossings compared to sevoflurane -anesthesia rats in the Morris water maze test. At the same time, the expression of LIMK1 was dramatically increased. CONCLUSION: Altogether, these findings suggested that miR-125b-5p inhibitor could protect against the sevoflurane -induced cognitive impairments by targeting LIMK1 . Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.
Entities: Chemical
Disease
Gene
Species
Keywords:
LIMK1; Morris Water Maze (MWM); SH-SY5Y; Sevoflurane; cognitive impairments; miR-125b-5p.
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Year: 2019
PMID： 31490755 DOI： 10.2174/1567202616666190906145936
Source DB: PubMed Journal: Curr Neurovasc Res ISSN： 1567-2026 Impact factor: 1.990