Bartłomiej Perek1, Katarzyna Kowalska2, Bartosz Kempisty2,3, Mariusz Nawrocki3, Michał Nowicki2, Mateusz Puślecki4, Danuta Ostalska-Nowicka5, Łukasz Szarpak6, Navid Ahmadi4, Agnieszka Malińska2. 1. Department of Cardiac Surgery and Transplantology, Chair of Cardio-Thoracic Surgery, Poznan University of Medical Sciences, Poznan, Poland. bperek@ump.edu.pl. 2. Department of Histology and Embryology, Poznan University of Medical Sciences, Ul. Święcickiego6, 60-781 Poznań, Poland. 3. Department of Anatomy, Poznan University of Medical Sciences, Poland. 4. Department of Cardiac Surgery and Transplantology, Chair of Cardio-Thoracic Surgery, Poznan University of Medical Sciences, Poznan, Poland. 5. Department of Pediatric Nephrology, Poznan University of Medical Sciences, Poland. 6. Łazarski University, Warszawa.
Abstract
BACKGROUND: Late failure of arterial aortocoronary conduits may result from abnormal activity of cells found in the vessel wall, including macrophages. The purpose of this study was to assess if there are any associations between the number of macrophages and overexpression of matrix metalloproteinases (MMPs) in the wall of arterial grafts, as well as their clinical significance. METHODS: This study involved 128 consecutive patients with a mean age of 64.9 ± 9.7 years who underwent elective surgery for coronary artery disease (CAD). The surplus segments of internal thoracic artery (ITA) and radial arteries (RA) were taken for immunohistochemical analysis of macrophage numbers and MMPs expression. The participants who reached the clinical primary end-point (cardiacrelated death, acute coronary syndrome or progression of CAD) had a follow-up angiography. RESULTS: The mean numbers of macrophages were higher on RA (70 [24; 112]) than ITA cross-sections (44 [24; 59]; p < 0.001). Median expression of both MMP2 and MMP9 were stronger in the ITA than RA cross-sections (p < 0.001). A significant positive correlation of MMP2 expression and a number of macrophages infiltrating the tunica media of arterial segments were noted on both ITA and RA cross-sections. In addition, the arterial segments of the 6 patients who reached clinical end-point had higher numbers of macrophages and stronger MMP2 expression when compared to the rest of the participants. CONCLUSIONS: Macrophage infiltration of arterial wall grafts prior to harvesting may be associated with higher risk of late occlusion and MMP2 might be facilitating this process.
BACKGROUND: Late failure of arterial aortocoronary conduits may result from abnormal activity of cells found in the vessel wall, including macrophages. The purpose of this study was to assess if there are any associations between the number of macrophages and overexpression of matrix metalloproteinases (MMPs) in the wall of arterial grafts, as well as their clinical significance. METHODS: This study involved 128 consecutive patients with a mean age of 64.9 ± 9.7 years who underwent elective surgery for coronary artery disease (CAD). The surplus segments of internal thoracic artery (ITA) and radial arteries (RA) were taken for immunohistochemical analysis of macrophage numbers and MMPs expression. The participants who reached the clinical primary end-point (cardiacrelated death, acute coronary syndrome or progression of CAD) had a follow-up angiography. RESULTS: The mean numbers of macrophages were higher on RA (70 [24; 112]) than ITA cross-sections (44 [24; 59]; p < 0.001). Median expression of both MMP2 and MMP9 were stronger in the ITA than RA cross-sections (p < 0.001). A significant positive correlation of MMP2 expression and a number of macrophages infiltrating the tunica media of arterial segments were noted on both ITA and RA cross-sections. In addition, the arterial segments of the 6 patients who reached clinical end-point had higher numbers of macrophages and stronger MMP2 expression when compared to the rest of the participants. CONCLUSIONS: Macrophage infiltration of arterial wall grafts prior to harvesting may be associated with higher risk of late occlusion and MMP2 might be facilitating this process.
Authors: Mario Gaudino; Umberto Benedetto; Stephen Fremes; Giuseppe Biondi-Zoccai; Art Sedrakyan; John D Puskas; Gianni D Angelini; Brian Buxton; Giacomo Frati; David L Hare; Philip Hayward; Giuseppe Nasso; Neil Moat; Miodrag Peric; Kyung J Yoo; Giuseppe Speziale; Leonard N Girardi; David P Taggart Journal: N Engl J Med Date: 2018-04-30 Impact factor: 91.245
Authors: Ji Hyung Chung; Hyun Ju Jeon; Sung-Yu Hong; Da Lyung Lee; Kyung Hye Lee; Soo Hyuk Kim; Ye Sun Han; Ichiro Manabe; Yury I Miller; Sang-Hak Lee Journal: PLoS One Date: 2012-02-21 Impact factor: 3.240