Jinfeng Li1, Lifeng Huang2, Shuzhen Wang1, Zhenyu Zhang3. 1. Department of Obstetrics and Gynecology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China. 2. Department of Surgical Intensive Care Unit, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China. 3. Department of Obstetrics and Gynecology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China. Electronic address: zhenyuzhang@ccmu.edu.cn.
Abstract
OBJECTIVES: Regulatory T cells (Tregs) and dendritic cell (DC) subsets play an essential role in the development of pregnancy immune tolerance. We aimed to investigate the proportion of CD4+CD25+FOXP3+ Tregs and DC subsets in peripheral blood of patients with pre-eclampsia compared to normal pregnant women. STUDY DESIGN: Peripheral blood samples were collected from 25 women with pre-eclampsia and 30 women with normal, healthy pregnancies. The ratio of CD4+CD25+ T cells in all CD4+ T cells, expression of FOXP3, and the ratio of myeloid DC (mDC) versus plasmacytoid DC (pDC) were assessed by flow cytometry. Levels of IL-10 and TGF-β in culture supernatants of purified CD4+CD25+ T cells were analyzed by enzyme-linked immunosorbent assay. RESULTS: The mean ratio of CD4+CD25+ T cells in CD4+ T lymphocytes in peripheral blood was lower in pre-eclampsia women than in normal pregnancies. The expression of Tregs marker FOXP3 in the pre-eclampsia group was significantly lower than the control group. The levels of IL-10 and TGF-β in supernatants of CD4+CD25+ T cells were significantly decreased in the pre-eclampsia group compared with the control group. The ratio of mDC/pDC was significantly higher in the pre-eclampsia group when compared with the control group. CONCLUSIONS: A decreased proportion and secretion of related inhibitory cytokines of CD4+CD25+FOXP3+ Tregs was found in the peripheral blood of pre-eclampsia, while the ratio of mDC/pDC increased. It is speculated that Tregs and DCs may play a role in the decreased immunosuppressive function of pre-eclampsia patients.
OBJECTIVES: Regulatory T cells (Tregs) and dendritic cell (DC) subsets play an essential role in the development of pregnancy immune tolerance. We aimed to investigate the proportion of CD4+CD25+FOXP3+ Tregs and DC subsets in peripheral blood of patients with pre-eclampsia compared to normal pregnant women. STUDY DESIGN: Peripheral blood samples were collected from 25 women with pre-eclampsia and 30 women with normal, healthy pregnancies. The ratio of CD4+CD25+ T cells in all CD4+ T cells, expression of FOXP3, and the ratio of myeloid DC (mDC) versus plasmacytoid DC (pDC) were assessed by flow cytometry. Levels of IL-10 and TGF-β in culture supernatants of purified CD4+CD25+ T cells were analyzed by enzyme-linked immunosorbent assay. RESULTS: The mean ratio of CD4+CD25+ T cells in CD4+ T lymphocytes in peripheral blood was lower in pre-eclampsia women than in normal pregnancies. The expression of Tregs marker FOXP3 in the pre-eclampsia group was significantly lower than the control group. The levels of IL-10 and TGF-β in supernatants of CD4+CD25+ T cells were significantly decreased in the pre-eclampsia group compared with the control group. The ratio of mDC/pDC was significantly higher in the pre-eclampsia group when compared with the control group. CONCLUSIONS: A decreased proportion and secretion of related inhibitory cytokines of CD4+CD25+FOXP3+ Tregs was found in the peripheral blood of pre-eclampsia, while the ratio of mDC/pDC increased. It is speculated that Tregs and DCs may play a role in the decreased immunosuppressive function of pre-eclampsia patients.
Authors: Shobana Navaneethabalakrishnan; Hannah L Smith; Cristina M Arenaz; Bethany L Goodlett; Justin G McDermott; Brett M Mitchell Journal: Am J Hypertens Date: 2022-10-03 Impact factor: 3.080