Literature DB >> 31486973

Flavin-Based Fluorescent Protein EcFbFP Auto-Guided Surface Display of Methyl Parathion Hydrolase in Escherichia coli.

Lu Bian1, Zhen Zhang1, Rong-Xing Tang1, Wei Shen1, Li-Xin Ma2.   

Abstract

Methyl parathion hydrolase (MPH) plays an important role in degrading a range of organophosphorus compounds. In order to display MPH on the cell surface of Escherichia coli strain RosettaBlue™, the Flavin-based fluorescent protein EcFbFP was severed as an auto-anchoring matrix. With net negative charges of EcFbFP supplying the driving forces, fusion protein MPH-EcFbFP through a two-step auto-surface display process was finally verified by (a) inner membrane translocation and (b) anchoring at outer membrane. Cells with surface-displayed MPH obtained activity of 0.12 U/OD600 against substrate methyl parathion. MPH when fused with engineered EcFbFP containing 20 net negative charges exhibited fivefold higher anchoring efficiency and tenfold higher enzymatic catalytic activity of 1.10 U/OD600. The above result showed that MPH was successfully displayed on cell surface and can be used for biodegradation of methyl parathion.

Entities:  

Keywords:  Cell-surface display; EcFbFP; Flavin-based fluorescent protein; Methyl parathion hydrolase; Net negative charge

Mesh：

Substances：

Year:  2019        PMID： 31486973     DOI： 10.1007/s12033-019-00204-3

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  39 in total

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Authors:  Dong Gyun Kang; Gio-Bin Lim; Hyung Joon Cha
Journal:  J Biotechnol       Date:  2005-09-10       Impact factor: 3.307

7.  Improved degradation of organophosphorus nerve agents and p-nitrophenol by Pseudomonas putida JS444 with surface-expressed organophosphorus hydrolase.

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Journal:  Biotechnol Prog       Date:  2005 May-Jun

8.  Optimization of a heterologous signal peptide by site-directed mutagenesis for improved secretion of recombinant proteins in Escherichia coli.

Authors:  Mohd Anuar Jonet; Nor Muhammad Mahadi; Abdul Munir Abdul Murad; Amir Rabu; Farah Diba Abu Bakar; Raha Abdul Rahim; Kheng Oon Low; Rosli Md Illias
Journal:  J Mol Microbiol Biotechnol       Date:  2012-03-23

9.  Immobilized Pseudomonas cepacia lipase for biodiesel fuel production from soybean oil.

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Journal:  Bioresour Technol       Date:  2005-05       Impact factor: 9.642

10.  Flavin mononucleotide-based fluorescent proteins function in mammalian cells without oxygen requirement.

Authors:  Janine Walter; Sascha Hausmann; Thomas Drepper; Michael Puls; Thorsten Eggert; Marcel Dihné
Journal:  PLoS One       Date:  2012-09-11       Impact factor: 3.240
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